LOCUS       sequence-81138-e        4839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti NPY-like receptor super contig 1.686 (NPYLR6) 
            full-length clone in mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Liesch J, Bellani LL, Vosshall LB
  TITLE     Functional and genetic characterization of neuropeptide Y-like 
            receptors in Aedes aegypti.
  JOURNAL   PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 
            10.1371/journal.pntd.0002486. eCollection 2013.
  PUBMED    24130914
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2013-10-10"; volume: "7(10):e2486. doi"; pages: "
            10.1371/journal.pntd.0002486. eCollection 2013"
COMMENT     Sequence Label: pME18S-sc1686
FEATURES             Location/Qualifiers
     source          1..4839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..363,364..632)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2744..2878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2781..2800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2835..2854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2917..2934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3088..3676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3168..3187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3847..4638,4639..4707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4470..4489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4708..4812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattcc tcgagatgga ttacccaatg atagcaacgg gcaacatctc
      841 gcaggaagac ttcctcgcca tagtcggggc gaactcttcg ctcgccagct taatccacgg
      901 atcggcaacg accgccctaa acgactccct gggcggagtg atagcaaccg gcgacaaccg
      961 aacactcttc cacggctacc cgagcggata cacactgccg cacatcatca tcgcttcaat
     1021 tatcgtgaca atactaatga taatcatcgt agtcggtaac atgctggtga tcatagcgat
     1081 tgccaccgaa aaggcgctca agaacatcca gaactggttc atagcatcgc tggcggtggc
     1141 ggacttcttt ctgggtctgg tgatcatgcc cttctcgttg gccaacgagc tgatgggcta
     1201 ctggatcttc gggaactggt ggtgcgacat ccactcggcg atggatgtgt tgttgtgtac
     1261 ttcgtcgatc atgaatctct gcctgatctc gctggatcgg tactggagca taacgaaggc
     1321 gatcgagtat ctcaaatcga ggactccggg ccgggcggct tttatgatcg ctgccgtgtg
     1381 gatcatgtcg gcactggtgt gcataccgcc gctgctgggc tggaaggcgc cacgtccgga
     1441 ggagcacgtc gaacttccac aatgtcagtt gagtcaagac attggctacg tgctgtactc
     1501 ggcgctcgga tcgttctaca ttcccagctg catcatggtg ttcgtctaca ttaggatcta
     1561 ctacgcggca aaggccaggg cccggcgggg gatccggaag aagccgctca aaccgcccag
     1621 cgagcaggac acaagcttca cgcgtccagc gaatccgatg ccatcgcttc ccagtgcctc
     1681 atcgatgtcg gccgttgcgg tggctccggc gtcatccgtc aacggaaacg gccaatacgc
     1741 cacggccaac agcaacaaca acaataacct aagtagtggc cccaacaatc ctggccagca
     1801 tcagcagcag atagcgacga tagaacctcc accgaggggg ctggcgggag gtgcagctgg
     1861 tggtccaacg cagcagatgt cgatcccgac ggtgacgtgc gacttcgcat ccgatatgtc
     1921 caccagcgaa gcagccgacc atgaccatgc cgctgtggta gcggagatga aggatacgct
     1981 caaagtcggt aaaccgggga tcgggctaac gtcactgcca ttgctgagag ggaacaacgg
     2041 ttcggcggcc agcagttcgg gggcggtgat tagtccgggg ttagctagga atagagcact
     2101 ttcggtaggg atcgaaaccg atatggttag cgaaattgat ccatcgagtt ccgactcggg
     2161 ggtgataagt aggtgtgcgg tggtgaaacc gctcaagttt aggctgtgtc aaccaatttt
     2221 cggcaagaga gtaggtaagg gtcgtaataa gaacaataat gcgaggggaa attgcgcagg
     2281 ggtgggaccg gcagcgatgg gaggtcaagc gatatcgata tcggccaaac agaacgatag
     2341 cgcttgtagc gaactggaac ctgcactgcc aaagccgaag ccacgggacc cggagaaaga
     2401 aaagcgacgg atagcgcgga agaaggagaa gcgagctaca ctgattctgg ggttaattat
     2461 gggaagtttt attgcctgct ggttgccatt tttcttcctt tacatattgg tgcccgtttg
     2521 caaggactgt cacataccgg actttgcttt ctcgttggcc ttttggttgg gttacatgaa
     2581 ctcgactctg aacccggcaa tctacaccat cttcaacaag gacttccgga gagccttcag
     2641 gcggatcctg ttcaagtgag cggccgctag actagtctag agaaaaaacc tcccacacct
     2701 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2761 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2821 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccc
     2881 cgggtaccga gctcgaatta attcctcttc cgcttcctcg ctcactgact cgctgcgctc
     2941 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3001 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3061 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3121 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3181 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     3301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3421 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3481 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3541 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3601 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3661 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3721 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3781 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3841 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3901 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3961 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4021 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4081 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4141 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4201 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4261 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4321 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4381 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4441 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4501 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4561 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4621 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4681 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4741 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4801 aggggttccg cgcacatttc cccgaaaagt gccacctgc
//
