LOCUS       sequence-81140-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PP1 beta residues 6-327.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     pp1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RP1B-PP1beta
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg ctctgataaa attcatcatc
       61 atcatcatca cgaaaatctt tattttcaag gtcatatgct gaacgtggac agcctcatca
      121 cccggctgct ggaagtacga ggatgtcgtc ctgggaagat tgttcagatg acagaagcag
      181 aagttcgggg cctgtgcata aaatcacgag aaatatttct tagccagcct attctcctgg
      241 aactagaggc accgctgaaa atctgtggtg atattcatgg tcagtataca gatttgctta
      301 gactgtttga atatggagga ttcccaccag aagcgaatta tcttttcctt ggagattatg
      361 tggacagagg caaacaatct ctggaaacaa tttgcctact tctggcatat aaaatcaagt
      421 acccggagaa cttctttctc ttaagaggaa atcatgagtg cgctagcatc aaccggatct
      481 atggattcta tgatgaatgt aagcggaggt ttaatattaa gctctggaag accttcacag
      541 actgctttaa ttgtctgcct attgcagcca ttgtggatga gaagatcttc tgctgtcatg
      601 gaggactgtc tccagatctc cagtcaatgg agcagatccg gagaattatg agacctacag
      661 atgttcctga cacaggctta ctatgtgacc tgctgtggtc tgacccagac aaagatgtgc
      721 aaggttgggg tgaaaatgat cgtggggtct cttttacttt cggtgctgat gtggtcagta
      781 aatttctgaa tcgtcacgat ttggatttaa tctgtcgagc tcaccaggtg gttgaagatg
      841 gatacgaatt ctttgctaaa cggcagttgg tcaccctatt ctcagctcca aattattgtg
      901 gagagtttga caatgcaggt ggcatgatga gtgtggacga aactctgatg tgttc
//
