LOCUS       sequence-81141-e        4152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti NPY-like receptor AAEL008296B (NPYLR7B) full-length 
            clone in mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Liesch J, Bellani LL, Vosshall LB
  TITLE     Functional and genetic characterization of neuropeptide Y-like 
            receptors in Aedes aegypti.
  JOURNAL   PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 
            10.1371/journal.pntd.0002486. eCollection 2013.
  PUBMED    24130914
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2013-10-10"; volume: "7(10):e2486. doi"; pages: "
            10.1371/journal.pntd.0002486. eCollection 2013"
COMMENT     Sequence Label: pME18S-8296B
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..363,364..632)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2057..2191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2094..2113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2148..2167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2230..2247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2401..2989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2481..2500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3160..3951,3952..4020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3783..3802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4021..4125)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattcc tcgaggcgat gaacttcact gccgagttct tcccggaaaa
      841 cgggactcaa ccatccgagg tcaacgcaac aaccttcgac tgcgacagtt cgtcctacat
      901 cccgcaagga atcgcctcgc tccgcttcca gatcctgatc tacctggcgt acagcgcgat
      961 cttcctgacc tcgatcgtcg gaaacgtggc cgtattcctc gtggtccact tgctaccccg
     1021 catgaacacc gtcaccaacc tcttcatcgc gaacctcgcc ctcggcgaca tgctgatgac
     1081 catcttctgc atcccgttct cgttcgtgtc gatcttcgtc ctgcagtact ggcccttcgg
     1141 cgcgatcatc tgccgcatcg ttaactactc ccaggccatc tcggtgctgg tcagtgccta
     1201 caccatgatc gcgatcagtg ccgatcgcta cctggcaatc atgtggcccc tcaaaccccg
     1261 ggtcaccaaa cgactcgctc ggatcctcat ctttctcgtt tggactggtg ccctcgcaac
     1321 cgctgcgccc atccccgtct tctccaccct gatccaaccg accgaattct acgatcactg
     1381 cgatctctcg atctgcaccg aggtgtggcc cgacgaccac tccgatcacg gctactccct
     1441 aaccctgatg accctccaat tcctcgcccc gttgatcgtc ctcatcacca cctacgtcca
     1501 gatcgcctgc aaagtctggg ccaagacccc tccgggcgaa tccgtcaaac agcgcgatca
     1561 acgcatcctc cagtccaagc gtaagatgat caagatgatg atcaccgtgg tggcggtctt
     1621 cacgatctgc tggctgccgt tcaacatctt catgctggtt ccgctggacc ccgactggcg
     1681 tccgctgccc tacctgtggt tcctgttcca ctggctggcg atgtcgcaca gctgctacaa
     1741 tccgatcatc tactgctaca tgaacgagaa gttccgacag gggttccagc ggctggtgga
     1801 gatgatcggg cgccggtgtt gctgctggat gggcgcgacg cggcggaaga gtgatgccag
     1861 taccaatacg gagatgctgc agttgttccc gagcacgacg ctgatgacca cggtggccag
     1921 tggcgttggg agtgctgcgg gaagtcgcga gtggtgccgg aaggggttgt aggcggccgc
     1981 tagactagtc tagagaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2041 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2101 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2161 actcatcaat gtatcttatc atgtctggat ccccgggtac cgagctcgaa ttaattcctc
     2221 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2281 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2341 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2401 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2461 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2521 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2581 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2641 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2701 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2761 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2821 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2881 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2941 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3001 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3061 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3301 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4081 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4141 agtgccacct gc
//
