LOCUS       sequence-81142-e        4218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti NPY-like receptor AAEL010626 (NPYLR8) full-length 
            clone in mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Liesch J, Bellani LL, Vosshall LB
  TITLE     Functional and genetic characterization of neuropeptide Y-like 
            receptors in Aedes aegypti.
  JOURNAL   PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 
            10.1371/journal.pntd.0002486. eCollection 2013.
  PUBMED    24130914
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2013-10-10"; volume: "7(10):e2486. doi"; pages: "
            10.1371/journal.pntd.0002486. eCollection 2013"
COMMENT     Sequence Label: pME18S-10626
FEATURES             Location/Qualifiers
     source          1..4218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..363,364..632)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2123..2257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2160..2179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2214..2233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2296..2313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2467..3055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2547..2566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3226..4017,4018..4086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3849..3868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4087..4191)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattcc tcgagatgga cgtggtcctg tccaggctga acatcagctt
      841 ggataatgtg acccttagca ccagcaccat ccggcagggg ctcatcgaag agtacagcaa
      901 caatcggaag gtcggcgagc ccgcctacca catactgatt gttctgtaca gcatactgat
      961 catatttggc gccacgggca acagtctggt ggtaatggcc gtcgctcgga aaccgcagat
     1021 gcgaacggcg cgcaacatgt tcatcgtcaa tttggccgtt tccgatttac tactgtgcct
     1081 ggttacgatg ccgctgacgc tggtggaaat tctcaccaaa tattggccca tgggtcggtt
     1141 gccgttcctg tgcaaatcga ttggcaccct tcaggccacc agcatcttcg tgtccaccat
     1201 ttccatcacg gccatcgcac tggaccgata tcaagtcatt gtctacccca ctcgtgacag
     1261 tctccagcgg atgggtgcca ttgtgatact aaccggcatc tggatcatct cgctggtgct
     1321 tgcctcgccg atgttcatca ccaggcggct gatccactac gatgtcaact tgcccagcct
     1381 ggggatcgag tatatttcct actgcatcga agactggcca atggcacgtg gccgagtgta
     1441 ttattcggtg ttcaccctat gcgtccagta tctcctgcca attctgatag tgtcgatagc
     1501 gtatttacga atttatctca aactgaagca ccgtctcgta gtaacgacca ctaccgtgag
     1561 caccaattcc aactcgatgg attcgcagcc cgtacgggaa cgggaacgag gccggcgaat
     1621 gcaacgaacc aactacttgc tgataagtat agccctaatc tttggaatct cgtggcttcc
     1681 actgaacctg ttcaacctgt ttgccgacct gtatctaagt ggaatcaccc aggagatcat
     1741 ggtggcatat gccatctgcc acatgatggg catgagctcg gcctgttcca atccgctact
     1801 ctacggttgg ctgaacgata actttcgtaa agagtttaac gagatcctct gccgaaagga
     1861 gtcaagttca ctcgttgcga acggtgccgg tggaggcaaa accatctcac gaaagattcg
     1921 aactacagga cccgctccgg aagtaacgca ggtgggcgtc ttgtgtcgac aaacgaccac
     1981 aagctcaacc gtagtagact cgggcgacca aaccgagctt accgagctca tgccgtgagc
     2041 ggccgctaga ctagtctaga gaaaaaacct cccacacctc cccctgaacc tgaaacataa
     2101 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2221 gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccgag ctcgaattaa
     2281 ttcctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2641 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2881 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4201 ccgaaaagtg ccacctgc
//
