LOCUS       sequence-81143-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PP1 gamma residues 7-323.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     pp1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RP1B-PP1gamma
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aattttgttt aactttaaga aggagatata ccatgggctc tgataaaatt catcatcatc
       61 atcatcacga aaatctttat tttcaaggtc atatgctcaa catcgacagc atcatccaac
      121 ggctgctgga agtgagaggg tccaagccag gcaagaatgt ccagctccag gagaatgaaa
      181 tccggggact gtgcttgaag tctcgggaga tcttcctcag tcagcctatc cttttagaac
      241 ttgaagcacc actcaagata tgtggtgaca tccacgggca gtactatgat ttgctccgtc
      301 tgtttgaata cggtggcttt cctccagaaa gcaactattt gtttctcggg gactatgtgg
      361 acaggggcaa acagtcacta gagacgatct gcctcttgct ggcctacaaa atcaagtatc
      421 cggagaactt ttttcttctt agagggaacc atgagtgtgc cagcatcaat agaatctacg
      481 gattttatga tgagtgtaag agaagataca acattaagct gtggaaaacg ttcacagact
      541 gttttaactg cttaccgata gcagccatcg tggacgagaa gatattctgc tgtcatggag
      601 gtttatcacc agatcttcaa tctatggagc agattcggcg aattatgaga ccaactgatg
      661 taccagatca aggtcttctt tgtgatcttt tgtggtctga ccccgataaa gatgtcttag
      721 gctggggtga aaatgacaga ggagtgtcct tcacatttgg tgcagaagtg gttgcaaaat
      781 ttctccataa gcatgatttg gatcttatat gtagagccca tcaggtggtt gaagatggat
      841 atgagtttt
//
