LOCUS       sequence-81159-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-GBP1-10gly-lexADBD
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(165..521,522..773))
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     complement(209..230)
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
       61 tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc
      121 caccaccttc tgataggcag cctgcacctg aggagtgcgg ccgcttacag ccagtcgccg
      181 ttgcgaataa ccccaaccgc cagaccttca atggtgaagc tctgctgacg aaggtcaacg
      241 acaattggtt taaactcgct attttctggc aacagttcga ctttattgcc ctgttttttc
      301 aggcgcttaa cggtaacttc gtcatcaata cgtgcgacaa cgacctgacc gttacgtaca
      361 tcctgagttt tatgcactgc cagcaagtca ccatccataa tgccgatatc tttcatcgac
      421 atcccgctga cgcgcagcag gaaatcagca ttcggcttga ataaggaagg atcgacctga
      481 taatgacctt caatatgctg ttgcgccaga agtggttcac cggcagccac acgacctacc
      541 agcggcaacc cttcttcctc ttcctgcaac agacgaatcc cgcgtgatgc gccggaaaca
      601 atttcaataa cgcctttgcg tgccagcgcc ttcagatgtt cttcagccgc gtttggggaa
      661 cggaacccca aacgctgcgc gatttccgca cgcgtcggcg gcatacctgt ctggctgatg
      721 tgatcacgga tgagatcaaa cacctcttgt tgcctggccg ttaacgcttt catactagta
      781 cctccacctc cacctccacc acctcctccg ctagcagaac taacagtcac ttgtgtgccc
      841 tgtccccagt attcaaagcc cacattgacg ttggagtagt acacagcagt gtcctcaggt
      901 ttcagggagt tcatctggag gtacacggtg tttctagcat cgtccctact aatggtga
//
