LOCUS       sequence-81160-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned 
            into pCI-neo).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Iwashita Y, Fukuchi N, Waki M, Hayashi K, Tahira T
  TITLE     Genome-wide repression of NF-kappaB target genes by transcription 
            factor MIBP1 and its modulation by O-linked beta-N-acetylglucosamine
            (O-GlcNAc) transferase.
  JOURNAL   J Biol Chem. 2012 Mar 23;287(13):9887-900. doi: 
            10.1074/jbc.M111.298521. Epub 2012 Jan 31.
  PUBMED    22294689
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.298521"; journalName: "J Biol Chem"; date: 
            "2012-03-23- 23"; volume: "287"; issue: "13"; pages: "9887-900"
COMMENT     Sequence Label: pCI-neo-FHM
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    25..73
                     /note="synthetic polyadenylation signal"
     CDS             complement(137..931)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(248..267)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     858..877
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgcggatcct tatcgctatc gattcacaca aaaaaccaac acacagatgt aatgaaaata
       61 aagatatttt attgcggcca tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
      121 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
      181 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
      241 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
      301 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
      361 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
      421 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
      481 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
      541 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
      601 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
      661 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
      721 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
      781 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
      841 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
      901 aacctgcgtg caatccatct tgttcaatca tggtggctct agccttaagt tcgagactgt
      961 tgtgtcagaa gaatcaagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     1021 cttctggaat agctcagagg cc
//
