LOCUS       sequence-81164-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cre recombinase under the control of the UAS-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pUAS-Cre
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..179
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        198..436
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     383..404
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      456..465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
      121 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      181 ggagactcta gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
      241 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
      301 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
      361 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
      421 caagaagaga actctgaata gggaattggg aattcgccac catggccaat ttactgaccg
      481 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
      541 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
      601 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
      661 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
      721 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
      781 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
      841 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
      901 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
      961 cttataacac cct
//
