LOCUS       sequence-81167-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tdTomato under the control of the TRE-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pTRE-tdTomato
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        102..416
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    110..128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    146..164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    181..199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    253..271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    288..306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    324..342
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     389..413
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      427..436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(566..584)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctggccggta cctgagctcg ctagcctcga ggatatcaag atctggcctc ggcggccaag
       61 cttgcatgcc tgcaggtcac gaggcccttt cgtcttcact cgagtttact ccctatcagt
      121 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac
      181 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      241 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga
      301 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc
      361 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      421 gaattcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
      481 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      541 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      601 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
      661 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      721 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
      781 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
      841 atg
//
