LOCUS       sequence-81181-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal 
            domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..159
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             166..186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcacc atggactaca aagaccatga cggtgattat
      121 aaagatcatg acatcgatta caaggatgac gatgacaaga tggcccccaa gaagaagagg
      181 aaggtgggca ttcaccgcgg ggtacctatg gtggacttga ggacactcgg ttattcgcaa
      241 cagcaacagg agaaaatcaa gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg
      301 cttgtggggc atggcttcac tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg
      361 cttgggacgg tggctgtcaa ataccaagat atgattgcgg ccctgcccga agccacgcac
      421 gaggcaattg taggggtcgg taaacagtgg tcgggagcgc gagcacttga ggcgctgctg
      481 actgtggcgg gtgagcttag ggggcctccg ctccagctcg acaccgggca gctgctgaag
      541 atcgcgaaga gagggggagt aacagcggta gaggcagtgc acgcctggcg caatgcgctc
      601 accggggccc ccttgaacct gaccccagac caggtagtcg caatcgcgtc aaacggaggg
      661 ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca agaccacggc
      721 cttacaccgg agcaagtcgt ggccattgca tcccacgacg gtggcaaaca ggctcttgag
      781 acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc cgatcaagtt
      841 gtagcgattg cgtc
//
