LOCUS       sequence-81182-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal 
            domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..50)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(66..653)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ccggtacgcg tagaatcgag accgaggaga gggttaggga taggcttacc ttcgaagggc
       61 ccttaaaagt ttatctcgcc gttattaaat ttccgtctca cttcctctaa ggttaatgtg
      121 ccggctttaa tcatttctcc accaattaaa agctcttcta cactaagaac agctccatta
      181 caattagtga tatgatttaa tcgtgtaagc tgagctttgt agtttccttt aaagtgacca
      241 ctcacaaata aaaacttaaa ttccgttaca gaagatggat agactttcca ccattcatta
      301 gggttgagat gtttgtttcg tgtttgattt tcttcgacat atcgctccat ttcatctgct
      361 tggccaattg gcagattata acctccgcta taagctttag tatccacgat cacaccgtaa
      421 tcaataggag atccgacagt ataaattgct ccgtccggtt tccttgatcc acccaaatgt
      481 ttacctctat atccataaac tttcataaaa aattccatta ccttcatttc aagaattcta
      541 tcctgagtgg aatttctggc aatttcaatt aattcaatat attcatgagg cacatatttc
      601 aatttatgac gaagttcaga tttcttctcc tccagttcac ttttgactag ttgggatccc
      661 gcgacttgat gggaagttct ctcgggaatc cgttggttgg ttctttggat caatgcggga
      721 gcatgaggca gacccttttt gactgcatcg agcgcgggtc gtccaccaag acatgccaac
      781 gccaccagat ggtcattcgt taacgcagcc aacgcgggat cgggcctgga caattgggct
      841 acgatggact ccaaggctgg ccgtcctccc ccat
//
