LOCUS       sequence-81184-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..154
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             161..181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcaccatgga ctacaaagac catgacggtg attataaaga
      121 tcatgacatc gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt
      181 gggcattcac cgcggggtac ctatggtgga cttgaggaca ctcggttatt cgcaacagca
      241 acaggagaaa atcaagccta aggtcaggag caccgtcgcg caacaccacg aggcgcttgt
      301 ggggcatggc ttcactcatg cgcatattgt cgcgctttca cagcaccctg cggcgcttgg
      361 gacggtggct gtcaaatacc aagatatgat tgcggccctg cccgaagcca cgcacgaggc
      421 aattgtaggg gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc tgctgactgt
      481 ggcgggtgag cttagggggc ctccgctcca gctcgacacc gggcagctgc tgaagatcgc
      541 gaagagaggg ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg cgctcaccgg
      601 ggcccccttg aacctgaccc cagaccaggt agtcgcaatc gcgtcacatg acgggggaaa
      661 gcaagccctg gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac
      721 accggagcaa gtcgtggcca ttgcaagcaa tgggggtggc aaacaggctc ttgagacggt
      781 tc
//
