LOCUS       sequence-81186-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..630)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gaggagaggg ttagggatag gcttaccttc gaagggccct taaaagttta tctcgccgtt
       61 attaaatttc cgtctcactt cctctaaggt taatgtgccg gctttaatca tttctccacc
      121 aattaaaagc tcttctacac taagaacagc tccattacaa ttagtcttat gatttaatcg
      181 tgtaagctga gctttgtagt ttcctttaaa gtgaccactc acaaataaaa acttaaattc
      241 cgttacagaa gatggataga ctttccacca ttcattaggg ttgatatgtt tgtttcgtgt
      301 ttgattttct ttgacatatc gttgcatttc atctgcttgg ccaattggca gattataacc
      361 tccgctataa gctttagtat ccacgatcac accgtaatca ataggagatc cgacagtata
      421 aattgctccg tccggtttcc ttgatccacc caaatgttta cctctatatc cataaacttt
      481 cataaaaaat tccattacct tcatttcaag aattctatcc tgagtggaat ttctggcaat
      541 ttcaattaat tcaatatatt catgaggcac atatttcaat ttatgacgaa gttcagattt
      601 cttctcctcc agttcacttt tgactagttg ggatcccgcg acttgatggg aagttctctc
      661 gggaatccgt tggttggttc tttggatcaa tgcgggagca tgaggcagac cctttttgac
      721 tgcatcgagc gcgggtcgtc caccaagaca tgccaacgcc accagatggt cattcgttaa
      781 cgcagcc
//
