LOCUS       sequence-81189-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermQ7
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..52)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(68..655)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaagg
       61 gcccttaaaa gtttatctcg ccgttattaa atttccgtct cacttcctct aaggttaatg
      121 tgccggcttt aatcatttct ccaccaatta aaagctcttc tacactaaga acagctccat
      181 tacaattagt gatatgattt aatcgtgtaa gctgagcttt gtagtttcct ttaaagtgac
      241 cactcacaaa taaaaactta aattccgtta cagaagatgg atagactttc caccattcat
      301 tagggttgag atgtttgttt cgtgtttgat tttcttcgac atatcgctcc atttcatctg
      361 cttggccaat tggcagatta taacctccgc tataagcttt agtatccacg atcacaccgt
      421 aatcaatagg agatccgaca gtataaattg ctccgtccgg tttccttgat ccacccaaat
      481 gtttacctct atatccataa actttcataa aaaattccat taccttcatt tcaagaattc
      541 tatcctgagt ggaatttctg gcaatttcaa ttaattcaat atattcatga ggcacatatt
      601 tcaatttatg acgaagttca gatttcttct cctccagttc acttttgact agttgggatc
      661 ccgcgacttg atgggaagtt ctctcgggaa tttgttggtt ggtttgttgg atcaatgcgg
      721 gagcatg
//
