LOCUS       sequence-81196-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..51)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(67..654)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaggg
       61 cccttaaaag tttatctcgc cgttattaaa tttccgtctc acttcctcta aggttaatgt
      121 gccggcttta atcatttctc caccaattaa aagctcttct acactaagaa cagctccatt
      181 acaattagtg atatgattta atcgtgtaag ctgagctttg tagtttcctt taaagtgacc
      241 actcacaaat aaaaacttaa attccgttac agaagatgga tagactttcc accattcatt
      301 agggttgaga tgtttgtttc gtgtttgatt ttcttcgaca tatcgctcca tttcatctgc
      361 ttggccaatt ggcagattat aacctccgct ataagcttta gtatccacga tcacaccgta
      421 atcaatagga gatccgacag tataaattgc tccgtccggt ttccttgatc cacccaaatg
      481 tttacctcta tatccataaa ctttcataaa aaattccatt accttcattt caagaattct
      541 atcctgagtg gaatttctgg caatttcaat taattcaata tattcatgag gcacatattt
      601 caatttatga cgaagttcag atttcttctc ctccagttca cttttgacta gttgggatcc
      661 cgcgactcga tgggaagttc tctcgggaat ccgccggttg gttcttttga tcaatgcggg
      721 agcatgaggc agaccctttt tgactgcatc gagcgcgggt cgtccaccaa gacatgccaa
      781 cgccaccaga tggtcattcg ttaacgcagc caacgcggga tcgggcctgg acaattgggc
      841 tacgatggac
//
