LOCUS       sequence-81197-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN1
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             167..187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcac catggactac aaagaccatg acggtgatta
      121 taaagatcat gacatcgatt acaaggatga cgatgacaag atggccccca agaagaagag
      181 gaaggtgggc attcaccgcg gggtacctat ggtggacttg aggacactcg gttattcgca
      241 acagcaacag gagaaaatca agcctaaggt caggagcacc gtcgcgcaac accacgaggc
      301 gcttgtgggg catggcttca ctcatgcgca tattgtcgcg ctttcacagc accctgcggc
      361 gcttgggacg gtggctgtca aataccaaga tatgattgcg gccctgcccg aagccacgca
      421 cgaggcaatt gtaggggtcg gtaaacagtg gtcgggagcg cgagcacttg aggcgctgct
      481 gactgtggcg ggtgagctta gggggcctcc gctccagctc gacaccgggc agctgctgca
      541 gatcgcgaag agagggggag taacagcggt agaggcagtg cacgcctggc gcaatgcgct
      601 caccggggcc cccttgaacc tgaccccaga ccaggtagtc gcaatcgcgt cacatgacgg
      661 gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg gtcctttgtc aagaccacgg
      721 ccttacaccg ga
//
