LOCUS       sequence-81203-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN2
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..49)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(65..652)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaagggcc
       61 cttaaaagtt tatctcgccg ttattaaatt tccgtctcac ttcctctaag gttaatgtgc
      121 cggctttaat catttctcca ccaattaaaa gctcttctac actaagaaca gctccattac
      181 aattagtgat atgatttaat cgtgtaagct gagctttgta gtttccttta aagtgaccac
      241 tcacaaataa aaacttaaat tccgttacag aagatggata gactttccac cattcattag
      301 ggttgagatg tttgtttcgt gtttgatttt cttcgacata tcgctccatt tcatctgctt
      361 ggccaattgg cagattataa cctccgctat aagctttagt atccacgatc acaccgtaat
      421 caataggaga tccgacagta taaattgctc cgtccggttt ccttgatcca cccaaatgtt
      481 tacctctata tccataaact ttcataaaaa attccattac cttcatttca agaattctat
      541 cctgagtgga atttctggca atttcaatta attcaatata ttcatgaggc acatatttca
      601 atttatgacg aagttcagat ttcttctcct ccagttcact tttgactagt tgggatcccg
      661 cgactcgatg ggaagttctc tcgggaatcc gccggttggt tcttttgatc aatgcgggag
      721 catgaggcag accctttttg actgcatcga gcgcgggtcg tccaccaaga catgccaacg
      781 ccaccagatg gtcattcgtt aacgcagcca acgcgggatc gggcctggac aattgggcta
      841 cgatgg
//
