LOCUS       sequence-81206-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN2
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(85..672)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc cttaaaagtt tatctcgccg ttattaaatt tccgtctcac
      121 ttcctctaag gttaatgtgc cggctttaat catttctcca ccaattaaaa gctcttctac
      181 actaagaaca gctccattac aattagtctt atgatttaat cgtgtaagct gagctttgta
      241 gtttccttta aagtgaccac tcacaaataa aaacttaaat tccgttacag aagatggata
      301 gactttccac cattcattag ggttgatatg tttgtttcgt gtttgatttt ctttgacata
      361 tcgttgcatt tcatctgctt ggccaattgg cagattataa cctccgctat aagctttagt
      421 atccacgatc acaccgtaat caataggaga tccgacagta taaattgctc cgtccggttt
      481 ccttgatcca cccaaatgtt tacctctata tccataaact ttcataaaaa attccattac
      541 cttcatttca agaattctat cctgagtgga atttctggca atttcaatta attcaatata
      601 ttcatgaggc acatatttca atttatgacg aagttcagat ttcttctcct ccagttcact
      661 tttgactagt tgggatcccg cgactcgatg ggaagttctc tcgggaatcc gccggttggt
      721 tcttttgatc aatgcgggag catgaggcag accctttttg actgcatcga gcgcgggtcg
      781 tccaccaaga catgccaacg ccaccagatg gtcattcgtt aacgcagcca acgcgggatc
      841 gggcctggac aattgggcta cgatggactc caaggctggc cgtcctcccc cattagaagc
      901 aatggcgacc acctgttcgg gtgtcagtcc atgatcctgg cacagtacag gcagcaggcg
      961 ctgtactgtt tccagcgc
//
