LOCUS       sequence-81221-e        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-OTUD7B
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..38
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             51..77
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatctgccac catggactac aaggatgacg atgacaagct cgatggagga tacccatacg
       61 atgttccaga ttacgctgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatgt
      121 tctaccatac atccagctgg ctgaaagtac ttgaggatca catgaccctg gacatggatg
      181 ctgttctgtc agattttgtc cgttccacag gagcagagcc agggctagcg cgagatctcc
      241 tagaaggaaa gaattgggat gtgaatgccg ccctcagtga ttttgaacag ctacgtcaag
      301 tccatgctgg aaacctaccc ccatccttta gtgaggggag tggtggctcc aggacccctg
      361 aaaaagggtt ttctgacaga gagcctactc gccctccccg acccatcctc cagcggcagg
      421 atgacatcgt tcaagaaaaa cgcctgtcta ggggcatctc ccacgccagc tccagcattg
      481 tttccctggc ccggtcccat gtctcctcca atggtggggg tggggggagc aatgagcacc
      541 ccctggaaat gcccatctgt gccttccagc ttccagatct cactgtatac aatgaagact
      601 tccgcagctt catagagaga gacctcattg agcagtccat gctggttgcc ttggaacagg
      661 cagggcgttt gaactggtgg gtgagtgtgg atcccacctc tcagaggctg cttcctttgg
      721 caactactgg agatgggaac tgcctcctgc atgcagcctc ccttggaatg tggggtttcc
      781 atgatcggga cttgatgctg cggaaagctt tgtatgcact gatggagaag ggagttgaga
      841 aggaagcgtt gaaaaggcgc tggaggtggc agcagacaca gcagaataaa gagtcagggc
      901 tggtatacac agaagatgaa tggcagaagg agtggaatga actgatcaag cttgcctcaa
      961 gtgaaccccg aatgcatcta ggtaccaatg gagccaactg tggtggggtg gagagttctg
     1021 aggagcctgt atatgagagc cttgaagagt ttcacgtctt tgtccttgct catgtgctta
     1081 ggaggcccat a
//
