LOCUS       sequence-81223-e        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of hTERT. Used to create immortalized cell 
            lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp 
            PM, Sedivy JM, Weinberg RA
  TITLE     Dissociation among in vitro telomerase activity, telomere 
            maintenance, and cellular immortalization.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.
  PUBMED    9843956
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14723-8"
COMMENT     Sequence Label: pBABE-neo-hTERT
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(141..160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(794..813)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tccccccttt ttctggagac taaataaaat cttttatttt atcgatgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg taaccaccac acccgccgca tcgatgcatc
      661 gatgcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
      721 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
      781 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
      841 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
      901 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
      961 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     1021 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     1081 atgggcaggt agccggatca agcgtatgca gccgccgca
//
