LOCUS       sequence-81226-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: Transgene Module (TrZ)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..76
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     complement(477..498)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(738..757)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aactacctac agagatttaa agctctaagg taaatataaa atttactagg ttgtggaatt
       61 gtgagcgctc acaattccac agtcgaccct aggttgtgga gtgttaaact acggatccgt
      121 ctcccattag gcctacaatg gtgagacaag tagccaacag ggaagggttg caaatatcat
      181 ttgggcacac ctatgataat attgatgaag cagacagtat tcagcaagta actgagaggt
      241 gggaagctca aagccaaagt cctaatgtgc agtcaggtga atttattgaa aaatttgagg
      301 ctcctggtgg tgcaaatcaa agaactgctc ctcagggatc ctaattgttt gtgtatttta
      361 gattccaacc aagcttgcgg ccgctcagga gctaaggaat tgatcctcta gagtcgacct
      421 gcagcacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      481 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      541 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      601 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
      661 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      721 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      781 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gac
//
