LOCUS       sequence-81228-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used in phosphoprotein synthesis. Replace the sfGFP with gene of 
            interest (GOI). Add TAG amber codon(s) at the position(s) in GOI 
            where phosphoserine incorporation is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  TITLE     Rinehart Lab Phosphoprotein synthesis components
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(83..706)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     614..633
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
       61 ctactgccgc caggcatcta gattaagacc cactttcaca tttaagttgt ttttctaatc
      121 cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata
      181 attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt
      241 ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag
      301 cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt
      361 gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc
      421 catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt
      481 gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg
      541 ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta
      601 cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca
      661 gctctaatgc gctgttaatc actttacttt tatctaatct ggacatgtcg a
//
