LOCUS       sequence-81229-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used in phosphoprotein synthesis. Replace the sfGFP with gene of 
            interest (GOI). Add TAG amber codon(s) at the position(s) in GOI 
            where phosphoserine incorporation is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     Rinehart Lab Phosphoprotein synthesis components
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(44..754,755..757))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cagccggatc aagctttcaa tgatgatgat gatgatgtga gcctttatac agttcgtcca
       61 taccgtgcgt gatgccggcc gcggtcacga attccagcag gaccatgtgg tcacgctttt
      121 cgttcggatc tttactcagc acagactgcg tgctcaggta atgattgtcc ggcagcagga
      181 ccgggccatc accaatcggg gtgttttgct gatagtggtc ggccagttga acgctgccat
      241 cttccacatt atggcggatc ttgaagttag ccttgatacc gttcttctgc ttatctgcgg
      301 tgatgtacac gttgtgcgaa ttgaagttat attccagttt atggcccaga atattaccgt
      361 cttccttaaa atcgatgcct ttcagttcaa tacggttaac cagcgtatca ccttcgaatt
      421 tcacttcggc gcgcgttttg taggtgccgt catctttaaa tgagatggta cgttcttgga
      481 cataaccttc cggcatcgcg gacttgaaaa agtcgtggcg tttcatgtga tccgggtaac
      541 gactgaaaca ctgaacacca tacgtcagcg tggtgaccag ggtcggccac ggaaccggca
      601 gtttacccgt ggtgcaaata aacttcagcg tcagtttacc attggttgca tcgccttcac
      661 cttcgccgcg cacgctgaat ttgtgaccat tgacatcacc atccagttcc accagaatcg
      721 gcaccacgcc cgtaaacagt tcttcgccct tgctcatggt acctttctcc tctt
//
