LOCUS       sequence-81230-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  binds to Jnk.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Dickens M, Rogers JS, Cavanagh J, Raitano A, Xia Z, Halpern JR, 
            Greenberg ME, Sawyers CL, Davis RJ
  TITLE     A cytoplasmic inhibitor of the JNK signal transduction pathway.
  JOURNAL   Science. 1997 Aug 1. 277(5326):693-6.
  PUBMED    9235893
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Aug 1. 277(5326):693-6."
COMMENT     Sequence Label: pCDNA3 Flag Jip1 JBD
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..123
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(652..670)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(653..670)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(690..707)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    696..920
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttcggca gccaacatgg actataagga cgatgatgac
      121 aaacccaaag cggagtccaa ccaggatccg gcgcctcgca gccagggcca gggcccgggc
      181 acaggcagcg gagacaccta ccgacccaag aggcctacca cgctcaacct tttcccgcag
      241 gtgccgcggt ctcaggacac gctgaataat aactctttag gcaaaaagca cagttggcag
      301 gaccgtgtgt ctcgatcatc ctcccctctg aagacaggag aacagacgcc tccacatgaa
      361 cacatctgcc tgagtgatga gctgccaccc cagggcagtc ctgttcccac ccaggaccgc
      421 ggcacttcca ccgacagccc ttgtcgccga agtgcagcca cccagatggc acctccaagc
      481 ggtccccctg ccactgcgcc tggtggccgg ggccactccc atcgagaccg aatccactac
      541 caggcagatg tgcggctcga ggcgactgag gagatctacc tgaccccatg agaattctgc
      601 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta ttctatagtg
      661 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      721 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      781 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      841 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      901 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
      961 tccccacgcg ccctgtagcg g
//
