LOCUS       sequence-81247-e         429 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To generate transgenic axolotls using Tol2 or SceI mediated 
            transgenics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Khattak S, Murawala P, Andreas H, Kappert V, Schuez M, 
            Sandoval-Guzman T, Crawford K, Tanaka EM
  TITLE     Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, 
            metamorphosis, transgenesis and tamoxifen-mediated recombination.
  JOURNAL   Nat Protoc. 2014 Mar;9(3):529-40. doi: 10.1038/nprot.2014.040. Epub 
            2014 Feb 6.
  PUBMED    24504478
REFERENCE   2  (bases 1 to 429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2014.040"; journalName: "Nat Protoc"; date: 
            "2014-03"; volume: "9"; issue: "3"; pages: "529-40"
COMMENT     Sequence Label: pBSII-SK-mTol2-MCS
FEATURES             Location/Qualifiers
     source          1..429
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(45..56,57..244)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     261..281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        263..281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     333..349
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(383..399)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(384..400)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacccagagg tgtaaagtac
       61 ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt ttactttact
      121 tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt tagaaaaaaa
      181 agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt tttggagatc
      241 acttgggccc ggctcgacgc gcaattaacc ctcactaaag ggaacaaaag ctggagctta
      301 gggataacag ggtaatccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc
      361 aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc attaccctgt
      421 tatccctag
//
