LOCUS       sequence-81248-e        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of Tol2 transposase using T7 Promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Khattak S, Murawala P, Andreas H, Kappert V, Schuez M, 
            Sandoval-Guzman T, Crawford K, Tanaka EM
  TITLE     Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, 
            metamorphosis, transgenesis and tamoxifen-mediated recombination.
  JOURNAL   Nat Protoc. 2014 Mar;9(3):529-40. doi: 10.1038/nprot.2014.040. Epub 
            2014 Feb 6.
  PUBMED    24504478
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2014.040"; journalName: "Nat Protoc"; date: 
            "2014-03"; volume: "9"; issue: "3"; pages: "529-40"
COMMENT     Sequence Label: T7-TPase
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcgtaggga taacagggta atgcgcgcgt aatacgactc actatagggc gaattgggta
       61 ccgggccccc cctcgaggtc gagtcacatc tattaccaca atgcacagca ccttgacctg
      121 gaaattaggg aaattataac agtcaatcag tggaagaaaa tggaggaagt atgtgattca
      181 tcagcagctg cgagcagcac agtccaaaat cagccacagg atcaagagca cccgtggccg
      241 tatcttcgcg aattcttttc tttaagtggt gtaaataaag attcattcaa gatgaaatgt
      301 gtcctctgtc tcccgcttaa taaagaaata tcggccttca aaagttcgcc atcaaaccta
      361 aggaagcata ttgagagaat gcacccaaat tacctcaaaa actactctaa attgacagca
      421 cagaagagaa agatcgggac ctccacccat gcttccagca gtaagcaact gaaagttgac
      481 tcagttttcc cagtcaaaca tgtgtctcca gtcactgtga acaaagctat attaaggtac
      541 atcattcaag gacttcatcc tttcagcact gttgatctgc catcatttaa agagctgatt
      601 agtacactgc agcctggcat ttctgtcatt acaaggccta ctttacgctc caagatagct
      661 gaagctgctc tgatcatgaa acagaaagtg actgctgcca tgagtgaagt tgaatggatt
      721 gcaaccacaa cggattgttg gactgcacgt agaaagtcat tcattggtgt aactgctcac
      781 tggatcaacc ctggaagtct tgaaagacat tccgctgcac ttgcctgcaa aagattaatg
      841 ggctctcata cttttgaggt actggccagt gccatgaatg atatccactc agagtatgaa
      901 atacgtgaca aggttgtttg cacaaccaca gacagtggtt ccaactttat gaaggctttc
      961 agagtttttg gtgtggaaaa caatgatatc gagactgagg caagaaggtg tgaaagtgat
     1021 gacactgatt ctgaaggctg tggtgagg
//
