LOCUS       sequence-81266-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses CD4 with C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
  TITLE     Sema4D localizes to synapses and regulates GABAergic synapse 
            development as a membrane-bound molecule in the mammalian 
            hippocampus.
  JOURNAL   Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
  PUBMED    24036351
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2013-11"; volume: "57:23-32. doi"; pages: " 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10"
COMMENT     Sequence Label: pCMV-CD4-FLAG
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..36)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgcggccgct cacttgtcat cgtcgtcctt gtagtcggcc gctccaatgg ggctacatgt
       61 cttctgaaac cggtgagggc actggcaggt cttcttctca ctgaggagtc tcttgatctg
      121 agacatccgc tctgcttggc gccttcggtg ccggcacctg acacagaaga agatgcctag
      181 cccaatgaaa agcaggaggc cggcgacgcc ccccagcaca atcagggcca ttggctgcac
      241 cggggtggac catgtgggca gaacctcgat gttggattcc agcaggacct gtcccgagtc
      301 actcagcaga cactgccaca tccccgcctc agggttcagc acccacaccg ccttctcccg
      361 cttcgagacc tttgcctcct tgttctccag tttcaagctc agcatcagct taggggaggt
      421 gggtccccac acctcacagg tcaaattttt ctggagctga gtggctctca tcaccaccag
      481 gttcacttcc tgatgcaact ttcctgtttt cgcttcaagg gccagggtga ggtttccaga
      541 gccagcatac tgaggcaagg cctggggcag ggtgaggtgg agcgggagct tcttgcccat
      601 ctggagctta gggtcctggg taacccgttt tacagacact tccttgttct tcaggtcaaa
      661 ggtgatccaa gacttggagg aggaagccct ctccgcctgc caccacagct cgccactgcc
      721 cgtcagcttt tcaactgtaa aggcgagtgg gaaggagaac tccacctgtt ccccctcttt
      781 cttatagact atgctggagg ccttctggaa agctagcacc acgatgtcta ttttgaactc
      841 caccttcttc tggttctgca agacagtgca tgtccaggtg ccactatcct ggagctccag
      901 ctga
//
