LOCUS       sequence-81269-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUC21 (Amp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUC21-NotI
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     145..161
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     145..161
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(175..194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(176..194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(201..218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(201..217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(210..232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(517..536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     649..670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(824..843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatatcgcc gtggcggccg ccagctgaag cttcgtacgc tgcaggtcga cggatccccg
       61 gggttaatta aggcgcgcca gatctgttta aacgagctcg aattcatcga tgatatcaga
      121 tccactagtg gcctatgcgg ccgctctaga actagtggat cgatccccaa ttcgccctat
      181 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      241 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      301 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      361 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
      421 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      481 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
      541 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggtggg ccatcgccct
      601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      661 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
      721 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
      781 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
      841 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      961 aga
//
