LOCUS       sequence-81279-e        1430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(282-470)
FEATURES             Location/Qualifiers
     source          1..1430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..399
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     350..370
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     444..463
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        444..462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             476..508
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1125..1143)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1126..1143)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1163..1180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1169..1280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtcaaatgg gtgagagtat ttaccggtaa actgaccaat ttggcagtac atcaaggtgt
       61 acatattccc aagtacgccc cctattgacg tcaatgacgg taaatggccc ggcctggcat
      121 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      181 atcgctatta ccatggtgat gcagttttgg cagtacatca atgggcgtgg atagcggttt
      241 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      301 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      361 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      421 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttatggc
      481 tagcatgact ggtggacagc aaatgggtgg atctggagga gtgcagaggc ccccagaccc
      541 tgcagaaccc acctccacct tcatgccacc cacggagagc cggatgtcag ttagctcgga
      601 tccagaccct gccgcttact ctgtaactgc ggggcggcca cacccctcca tcagtgaaga
      661 ggatgagggc ttcgactgcc tgtcatcccc agagcgagct gagccaccag gtggagggtg
      721 gcggggaagc ctcggggagc caccaccgcc tccacgggcc tcactgagct cggacaccag
      781 cgcactgtcc tacgactcgg tcaagtacac actggtggtg gatgaacatg cccagcttga
      841 gttggtgagc ctgcggccgt gctttggaga ttacagtgac gaaagcgact ctgccactgt
      901 ctatgacaac tgtgcctctg cctcctcgcc ctacgagtca gccattggtg aggagtatga
      961 ggaggcccct cagccccggc ctcccacctg cctctcagag gactccaccc cggatgagcc
     1021 tgatgtccac ttctctaaga agtttctgaa tgtcttcatg agtggccgct ctcgttcctc
     1081 cagtgcttag gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
     1141 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1201 tgcacctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct gggggggtgg
     1321 ggtggggcag gacagcaagg ggggaggact gcgaagacaa tagcatgcat gctagggcac
     1381 gcggtgagct ctatggctct ctgaggcgca tagaatcaac ttggacttct
//
