LOCUS       sequence-81284-e        1471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcDNA3 T7 Jip1b(471-707)
FEATURES             Location/Qualifiers
     source          1..1471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        87..290
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     241..261
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     335..354
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        335..353
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             367..399
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1159..1177)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1160..1177)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1197..1214)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1203..1427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
       61 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      121 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      181 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      241 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      301 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      361 aagcttatgg ctagcatgac tggtggacag caaatgggtg gatccggagg agagtccttt
      421 gggctgttct cctgcgtcat caatggggag gagcatgagc aaacccatcg ggctatattc
      481 aggtttgtgc ctcggcatga agatgaactt gagctggaag tggatgaccc cctgctggtg
      541 gagctgcagg cagaagacta ttggtatgag gcctataaca tgcgcaccgg agcccgcggg
      601 gtcttccctg cctactatgc cattgaggtc accaaggagc ctgagcacat ggcagccctt
      661 gccaaaaaca gcgactggat tgaccagttc cgggtgaagt tcctggggtc tgtccaggtt
      721 ccttatcaca agggcaatga tgtcctctgt gctgctatgc aaaagatcgc caccacccgc
      781 cggctcaccg tgcactttaa cccgccctcc agctgtgtcc ttgagatcag tgtcaggggt
      841 gtcaagatag gcgtcaaagc tgatgatgct ctggaggcca agggaaataa atgtagccac
      901 ttcttccagc taaagaacat ctctttctgt ggataccatc caaagaataa caagtacttt
      961 gggtttatca ctaagcaccc tgctgaccac cggtttgcct gccatgtctt tgtgtctgaa
     1021 gattccacca aagccctggc ggagtctgtg gggcgtgcat ttcagcagtt ctacaagcag
     1081 tttgtggagt atacctgtcc tacagaagat atctacttgg ataggcggcc gctcgagcat
     1141 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     1201 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     1261 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     1321 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     1381 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     1441 aagaaccagc tggggctcta gggggtatcc c
//
