LOCUS       sequence-81287-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson
            CL, Kiguchi K, Walker CL
  TITLE     Activity of TSC2 is inhibited by AKT-mediated phosphorylation and 
            membrane partitioning.
  JOURNAL   J Cell Biol. 2006 Apr 24. 173(2):279-89.
  PUBMED    16636147
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Apr 24. 173(2):279-89."
COMMENT     Sequence Label: pcDNA3.1 myc TSC1
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..74)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtttaactc aatgatgatg atgatgatgg tcgacggcgc tattcagatc ctcttctgag
       61 atgagttttt gttcgggccc aagcttggta ccgctgtgtt catgatgagt ctcattgtag
      121 tccatgatat gtagctgtcc aacactgtcc ggggtcgggg gagacgggtg agggccatct
      181 aggttcaggg gaatcttggc ttccacaccc aagtctttgc ccagttctgt ctttaggctc
      241 tcagaaaggc tactggtcat gccgtcctca tcacactggc tctcgctctt attacgaaat
      301 aactctcgag ccttcatacc caggaagctt tttgaactgg gaagtgagcc cacagtggtg
      361 gggatgctgg cagacgcttc tcccatagtc gtctcccacc gactgctgaa tgggcctgcc
      421 ctctggtgtg ggggtttctc tggggtagaa agctcgctgc tgctgctgct gcctccacca
      481 cctctgcttc cactactgcc ccgggcgctg ctgggcctgg gggtcttggt ctcaccgttg
      541 tggccagatg cctcttcatt gtgccctacc atggaatctg agcacccgtc attacaacag
      601 tcaagccttt cttctgctgc ttcagctgct tctgcttttt cttcttcaag ttttttcagg
      661 aggccatctt tctccaacct gccatataaa tctaagatct ccaattcaaa cacctgggtt
      721 atccttttct gagcctcata cctgctctct gcggcctgca gctgtcctct tgcctggagt
      781 ttgacatcct ctagatattt cttctgttcc aaaagaaggt ggtctttctt ggccaggtga
      841 gattccagtt ccaaaatccg tttttgggag gtatcaagcc tctgagtctg ctggagaaca
      901 tggcttctgt ttttttctag ctctttccga taggcggctt tcatcatttc tacttccttt
      961 gtggtatctg a
//
