LOCUS       sequence-81297-e        2816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(C631S, C643S and C699T)
FEATURES             Location/Qualifiers
     source          1..2816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..230
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     181..201
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     275..294
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        275..293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             307..339
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(2504..2522)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2505..2522)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2542..2559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2548..2772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
       61 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      121 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      181 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      241 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      301 aagcttatgg ctagcatgac tggtggacag caaatgggtg gatccatggc ggagcgagag
      361 agcggcctgg gcgggggcgc cgcgtcccca ccggccgctt ccccattcct gggactgcac
      421 atcgcgtcgc ctcccaattt caggctcacc catgacatca gcctggagga gtttgaggat
      481 gaagaccttt cggagatcac tgacgagtgt ggcatcagcc tgcagtgcaa agacaccctg
      541 tctctccggc ccccgcgcgc cgggctgctg tctgcgggta gcagcggcag cgcggggagc
      601 cggctgcagg cggagatgct gcagatggac ctgatcgacg cggcaggtga cactccgggc
      661 gccgaggacg acgaggagga ggaggacgac gagctcgctg cccaacgacc aggagtgggg
      721 cctcccaaag cggagtccaa ccaggatccg gcgcctcgca gccagggcca gggcccgggc
      781 acaggcagcg gagacaccta ccgacccaag aggcctacca cgctcaacct tttcccgcag
      841 gtgccgcggt ctcaggacac gctgaataat aactctttag gcaaaaagca cagttggcag
      901 gaccgtgtgt ctcgatcatc ctcccctctg aagacaggag aacagacgcc tccacatgaa
      961 cacatctgcc tgagtgatga gctgccaccc cagggcagtc ctgttcccac ccaggaccgc
     1021 ggcacttcca ccgacagccc ttgtcgccga agtgcagcca cccagatggc acctccaagc
     1081 ggtccccctg ccactgcgcc tggtggccgg ggccactccc atcgagaccg aatccactac
     1141 caggcagatg tgcggctcga ggcgactgag gagatctacc tgaccccagt gcagaggccc
     1201 ccagaccctg cagaacccac ctccaccttc atgccaccca cggagagccg gatgtcagtt
     1261 agctcggatc cagaccctgc cgcttactct gtaactgcgg ggcggccaca cccctccatc
     1321 agtgaagagg atgagggctt cgactgcctg tcatccccag agcgagctga gccaccaggt
     1381 ggagggtggc ggggaagcct cggggagcca ccaccgcctc cacgggcctc actgagctcg
     1441 gacaccagcg cactgtccta cgactcggtc aagtacacac tggtggtgga tgaacatgcc
     1501 cagcttgagt tggtgagcct gcggccgtgc tttggagatt acagtgacga aagcgactct
     1561 gccactgtct atgacaactg tgcctctgcc tcctcgccct acgagtcagc cattggtgag
     1621 gagtatgagg aggcccctca gccccggcct cccacctgcc tctcagagga ctccaccccg
     1681 gatgagcctg atgtccactt ctctaagaag tttctgaatg tcttcatgag tggccgctct
     1741 cgttcctcca gtgctgagtc ctttgggctg ttctcctgcg tcatcaatgg ggaggagcat
     1801 gagcaaaccc atcgggctat attcaggttt gtgcctcggc atgaagatga acttgagctg
     1861 gaagtggatg accccctgct ggtggagctg caggcagaag actattggta tgaggcctat
     1921 aacatgcgca ccggagcccg cggggtcttc cctgcctact atgccattga ggtcaccaag
     1981 gagcctgagc acatggcagc ccttgccaaa aacagcgact ggattgacca gttccgggtg
     2041 aagttcctgg ggtctgtcca ggttccttat cacaagggca atgatgtcct ctgtgctgct
     2101 atgcaaaaga tcgccaccac ccgccggctc accgtgcact ttaacccgcc ctccagcagt
     2161 gtccttgaga tcagtgtcag gggtgtcaag ataggcgtca aagctgatga tgctctggag
     2221 gccaagggaa ataaaagtag ccacttcttc cagctaaaga acatctcttt cagtggatac
     2281 catccaaaga ataacaagta ctttgggttt atcactaagc accctgctga ccaccggttt
     2341 gccagccatg tctttgtgtc tgaagattcc accaaagccc tggcggagtc tgtggggcgt
     2401 gcatttcagc agttctacaa gcagtttgtg gagtatacca ctcctacaga agatatctac
     2461 ttggagtagg cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
     2521 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2761 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccc
//
