LOCUS       sequence-81298-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             108..140
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(895..913)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(896..913)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(933..950)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    939..1163
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
       61 ctggcttatc gaaattaata cgactcacta tagggagacc caagcttatg gctagcatga
      121 ctggtggaca gcaaatgggt ggatccgagt cctttgggct gttctccagc gtcatcaatg
      181 gggaggagca tgagcaaacc catcgggcta tattcaggtt tgtgcctcgg catgaagatg
      241 aacttgagct ggaagtggat gaccccctgc tggtggagct gcaggcagaa gactattggt
      301 atgaggccta taacatgcgc accggagccc gcggggtctt ccctgcctac tatgccattg
      361 aggtcaccaa ggagcctgag cacatggcag cccttgccaa aaacagcgac tggattgacc
      421 agttccgggt gaagttcctg gggtctgtcc aggttcctta tcacaagggc aatgatgtcc
      481 tctgtgctgc tatgcaaaag atcgccacca cccgccggct caccgtgcac tttaacccgc
      541 cctccagctg tgtccttgag atcagtgtca ggggtgtcaa gataggcgtc aaagctgatg
      601 atgctctgga ggccaaggga aataaaagta gccacttctt ccagctaaag aacatctctt
      661 tctgtggata ccatccaaag aataacaagt actttgggtt tatcactaag caccctgctg
      721 accaccggtt tgcctgccat gtctttgtgt ctgaagattc caccaaagcc ctggcggagt
      781 ctgtggggcg tgcatttcag cagttctaca agcagtttgt ggagtatacc actcctacag
      841 aagatatcta cttggagtag gcggccgctc gagcatgcat ctagagggcc ctattctata
      901 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
      961 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1021 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1081 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     1141 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     1201 gtatccc
//
