LOCUS       sequence-81299-e        1163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)
FEATURES             Location/Qualifiers
     source          1..1163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..70
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     21..41
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     115..134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        115..133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             147..179
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(934..952)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(935..952)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(972..989)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    978..1089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
       61 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      121 gactcactat agggagaccc aagcttatgg ctagcatgac tggtggacag caaatgggtg
      181 gatccgagtc ctttgggctg ttctccagcg tcatcaatgg ggaggagcat gagcaaaccc
      241 atcgggctat attcaggttt gtgcctcggc atgaagatga acttgagctg gaagtggatg
      301 accccctgct ggtggagctg caggcagaag actattggta tgaggcctat aacatgcgca
      361 ccggagcccg cggggtcttc cctgcctact atgccattga ggtcaccaag gagcctgagc
      421 acatggcagc ccttgccaaa aacagcgact ggattgacca gttccgggtg aagttcctgg
      481 ggtctgtcca ggttccttat cacaagggca atgatgtcct ctgtgctgct atgcaaaaga
      541 tcgccaccac ccgccggctc accgtgcact ttaacccgcc ctccagctgt gtccttgaga
      601 tcagtgtcag gggtgtcaag ataggcgtca aagctgatga tgctctggag gccaagggaa
      661 ataaatgtag ccacttcttc cagctaaaga acatctcttt cagtggatac catccaaaga
      721 ataacaagta ctttgggttt atcactaagc accctgctga ccaccggttt gcctgccatg
      781 tctttgtgtc tgaagattcc accaaagccc tggcggagtc tgtggggcgt gcatttcagc
      841 agttctacaa gcagtttgtg gagtatacca ctcctacaga agatatctac ttggagtagg
      901 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
      961 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1021 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1081 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     1141 cagcaagggg gaggattggg aag
//
