LOCUS       sequence-81300-e        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..232
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     183..203
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     277..296
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        277..295
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             309..341
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1096..1114)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1097..1114)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1134..1151)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1140..1366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
       61 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      121 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      181 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
      241 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      301 ccaagcttat ggctagcatg actggtggac agcaaatggg tggatccgag tcctttgggc
      361 tgttctccag cgtcatcaat ggggaggagc atgagcaaac ccatcgggct atattcaggt
      421 ttgtgcctcg gcatgaagat gaacttgagc tggaagtgga tgaccccctg ctggtggagc
      481 tgcaggcaga agactattgg tatgaggcct ataacatgcg caccggagcc cgcggggtct
      541 tccctgccta ctatgccatt gaggtcacca aggagcctga gcacatggca gcccttgcca
      601 aaaacagcga ctggattgac cagttccggg tgaagttcct ggggtctgtc caggttcctt
      661 atcacaaggg caatgatgtc ctctgtgctg ctatgcaaaa gatcgccacc acccgccggc
      721 tcaccgtgca ctttaacccg ccctccagct gtgtccttga gatcagtgtc aggggtgtca
      781 agataggcgt caaagctgat gatgctctgg aggccaaggg aaataaatgt agccacttct
      841 tccagctaaa gaacatctct ttctgtggat accatccaaa gaataacaag tactttgggt
      901 ttatcactaa gcaccctgct gaccaccggt ttgccagcca tgtctttgtg tctgaagatt
      961 ccaccaaagc cctggcggag tctgtggggc gtgcatttca gcagttctac aagcagtttg
     1021 tggagtatac cactcctaca gaagatatct acttggagta ggcggccgct cgagcatgca
     1081 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     1141 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1201 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1261 gagtaggtgt cattctattc tggggggtgg gggtggggca ggacagcaag gggggaggat
     1321 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     1381 agaaccagct ggggctctag ggg
//
