LOCUS       sequence-81313-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-AcrR
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(732..755)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(765..782)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             801..823
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    838..862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(862..881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(863..881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(965..984)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 gcggccgcaa gcttggatcc ttattcattg gttgccggat tacgcagggt cggacacagc
      121 agatacattt ccagcagaat tgcaacgtaa tcgcgtgctt cttttttcag atcgaatgac
      181 tgcggtgcaa acagccaatt ttccatcaga ccgctaatat aaccacgcat gataattgct
      241 gcacgacggg tcatcagatc tgcaggcagc atttttgctt caatgcaatg tttcagggtc
      301 tgttcaatac gatcatagct ttccagacac agattacgct gtgcctgctg aacaactgcc
      361 atttcaccca cgaactcgca tttgtgaaaa atgatttcca tcagcagacg acgacgttct
      421 tcggtaacgg tgctttccag aacatgaatc agaatttcac gcagaacgct cagcggatca
      481 cccggaaatt ttgcctggta ttccagttcc agttcaccaa tattgctttc gctcagttcc
      541 caaatttcgc taaacaggtc gcttttatct ttgaagtgcc aataaattgc accacgggta
      601 acaccggctg cttttgcaat ttcacccagg ctggtgctgc taacaccctg ctggctaaac
      661 agacgcagtg caacatccag aatatgctga cgggtttctt gtgcttcttg tttggtttta
      721 cgtgccatat ggggcccctg gaacagaact tccaggccgc tgctgtgatg atgatgatga
      781 tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt ctagagggga
      841 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      901 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      961 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     1021 a
//
