LOCUS       sequence-81320-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=300nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_300/pcDNA3
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(80..82,83..85,86..598)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(125..146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(386..405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
       61 ggtaccgagc tcggatccga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
      541 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
      601 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggggta ccaaggtgta
      781 cgaccccgag cagaggaaga ggatgatcac cggcccc
//
