LOCUS       sequence-81322-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of rat LC3 fused to mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Kimura S, Noda T, Yoshimori T
  TITLE     Dissection of the autophagosome maturation process by a novel 
            reporter protein, tandem fluorescent-tagged LC3.
  JOURNAL   Autophagy. 2007;3(5):452-60.
  PUBMED    17534139
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2007"; volume: "3(5)"; pages: "452-60"
COMMENT     Sequence Label: pmRFP-LC3
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(493..915)
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
     primer_bind     complement(984..1007)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatca gttatctaga tccggtggat cccgggcccg cggtaccgtc gactgcagaa
      181 ttcgcaccat agttataaac cagggacatg acgacgtaca caacccacac acggcagcag
      241 tggggactta cacagtgtaa cataacattg ggggttagca gggacagctg catgcaggga
      301 gggtgggtgc tgtggggagc caggtcgaga ggtggctgtg ttggcttccg tccgtgacag
      361 aggcagctct cactgagatt ctcagacacg agtgagtgag tgaccgggga tggtctgagt
      421 gtcacagtgg gctccatgca ggtagcaggg agcagaggct tgctttagtt ggaagtggct
      481 gtatgtctgt cacaagcatg gctctcttcc tgttgctgtt gccttcagag ctgacatgta
      541 tgtaacagcc agtgctgtcc cgaacgtctc ctgggaggca tagaccatgt acaggaagcc
      601 gtcttcatct ctctcgctct cgtacacttc agagatgggt gtggacacac tcaccatgct
      661 gtgcccattc accaggagga agaaggcttg gttagcattg agctgcaggc gccttctaat
      721 tatcttgatg agttcgctca tattcacgtg atcaggtaca aggaacttgg tcttgtccag
      781 gacgggcagc tgcttctcac ccttgtatcg ctctataatc actgggatct tggtggggtg
      841 ctgctcccgg atgagccgga catcttccac tctttgttcg aagctccggc gctgtttgaa
      901 ggtcttctcg gacggtctag atctggcgcc ggtggagtgg cggccctcgg cgcgctcgta
      961 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttga tgtc
//
