LOCUS       sequence-81327-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  TITLE     Bornstein plasmids
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBT metaxin 2
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(73..174)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(194..212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        280..384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             569..947
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(603..622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga ccagaactac ctgtgaaatt
       61 aataacagac atactccaag ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa
      121 tagtcaccaa tgccctccct cttggccctc tccttttctt ttttcgaccg aattaattct
      181 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      241 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      301 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      361 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      421 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      481 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      541 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      601 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      661 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      721 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      781 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      841 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      901 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      961 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1021 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctga
//
