LOCUS       sequence-81346-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a 'caged' Ca2+ indicator for fluorescent 
            imaging at specific locations and time points.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Matsuda T, Horikawa K, Saito K, Nagai T
  TITLE     Highlighted Ca(2)(+) imaging with a genetically encoded 'caged' 
            indicator.
  JOURNAL   Sci Rep. 2013;3:1398. doi: 10.1038/srep01398.
  PUBMED    23474844
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1398. doi"; pages: " 10.1038/srep01398"
COMMENT     Sequence Label: PA-TNXL/pcDNA3
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(96..98,99..101,102..614)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(141..162)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(402..421)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     749..770
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttggta ccgagctcgg atccgatggt gagcaagggc gaggagctgt
      121 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      181 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      241 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc agctacggcg
      301 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      361 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      421 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      481 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      541 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      601 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      661 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccaccag tccgccctga
      721 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccc
      781 gcatgctcag cgaggagatg attgctgagt tcaaagctgc ctttgacatg tttgatgcgg
      841 acggtggtgg ggacatcagc accaaggagt tgggcacggt gatga
//
