LOCUS       sequence-8135-ex       12069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM
  TITLE     Regulation of beta-catenin signaling by the B56 subunit of protein 
            phosphatase 2A.
  JOURNAL   Science. 1999 Mar 26. 283(5410):2089-91.
  PUBMED    10092233
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1999 Mar 26. 283(5410):2089-91."
COMMENT     Sequence Label: V248 pCEP 4HA B56gamma3
FEATURES             Location/Qualifiers
     source          1..12069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      475..2264
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2570..4495)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4930..4948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5016..5120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5121..5189,5190..5981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5339..5358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6152..6740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6641..6660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7357..7502
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7542..8567
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7542..8567
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(8588..8607)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8632..8680
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9333..9712
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9713..9916
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9867..9887
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9913..9937
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9970..9996
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10012..10101
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    11660..11794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11684..11703)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11738..11757
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(11936..11958)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gccccgccgc cggacgaact aaacctgact acggcatctc tgccccttct tcgctggtac
       61 gaggagcgct tttgttttgt attcggggca gtgcatgtaa tcccttcagt tggttggtac
      121 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt
      181 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc
      241 tttcatcctg gagcagactt tgcatgctgt ggactgcaac acaacattgc ctttatgtgt
      301 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg
      361 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg
      421 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg
      481 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat
      541 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa
      601 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca
      661 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt
      721 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact
      781 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc
      841 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata
      901 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct
      961 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac
     1021 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga
     1081 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag
     1141 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa
     1201 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt
     1261 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac
     1321 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac
     1381 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg
     1441 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga
     1501 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct
     1561 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     1621 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     1681 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     1741 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     1801 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt
     1861 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     1921 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     1981 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     2041 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     2101 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata
     2161 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc
     2221 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgcatgct ggttgctccc
     2281 attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag
     2341 gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg
     2401 acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat
     2461 ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg
     2521 ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc
     2581 cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg
     2641 cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc
     2701 tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa
     2761 tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga
     2821 caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga
     2881 gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt
     2941 tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt
     3001 cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct
     3061 caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc
     3121 gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg
     3181 cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt
     3241 ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag
     3301 ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct
     3361 tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt
     3421 cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc
     3481 gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg
     3541 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     3601 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctcctgctc
     3661 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3721 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     3781 ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     3841 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct gcccctcctc
     3901 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     3961 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4021 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4081 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     4141 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc
     4201 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc
     4261 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc
     4321 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat
     4381 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt
     4441 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc
     4501 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc
     4561 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
     4621 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
     4681 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
     4741 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
     4801 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
     4861 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
     4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5341 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5641 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     6841 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     6901 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     6961 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     7021 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa
     7081 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     7141 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     7201 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     7261 tcggccgcca tgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc
     7321 ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc
     7381 ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc caggtccact
     7441 tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt
     7501 aacagcgtca acagcgtgcc gcagatcccg ggcaatgaga tatgaaaaag cctgaactca
     7561 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     7621 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     7681 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     7741 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     7801 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     7861 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     7921 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     7981 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     8041 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     8101 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     8161 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     8221 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     8281 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     8341 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     8401 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     8461 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     8521 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagggg agatggggga
     8581 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     8641 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     8701 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     8761 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     8821 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
     8881 tggggaatgg tttatggttc gtgggggtta ttattttggg cgttgcgtgg ggtctggtcc
     8941 acgactggac tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt
     9001 actggcgcga cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac
     9061 caccgcgcgg atttctggcg tgccaagcta gtcgaccaat tctcatgttt gacagcttat
     9121 catcgcagat ccgggcaacg ttgttgcatt gctgcaggcg cagaactggt aggtatggaa
     9181 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     9241 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     9301 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
     9361 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     9421 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     9481 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     9541 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     9601 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     9661 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     9721 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     9781 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9841 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     9901 ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg taccacgcgt
     9961 catatggctt acccatacga tgttccagat tacgctagcg gccgcatctt ttacccatac
    10021 gatgttcctg actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca
    10081 tatgacgttc cagattacgc tgctcagtgc ggccgcgtgg tggatgcggc caactccaat
    10141 gggcctttcc agcccgtggt ccttctccat attcgagatg ttcctcctgc tgatcaagag
    10201 aagcttttta tccagaagtt acgtcagtgt tgcgtcctct ttgactttgt ttctgatcca
    10261 ctaagtgacc taaagtggaa ggaagtaaaa cgagctgctt taagtgaaat ggtagaatat
    10321 atcacccata atcggaatgt gatcacagag cctatttacc cagaagtagt ccatatgttt
    10381 gcagttaaca tgtttcgaac attaccacct tcctccaatc ctacgggagc ggaatttgac
    10441 ccggaggaag atgaaccaac gttagaagca gcctggcctc atctacagct tgtttatgaa
    10501 tttttcttaa gatttttaga gtctccagat ttccaaccta atatagcgaa gaaatatatt
    10561 gatcagaagt ttgtattgca gcttttagag ctctttgaca gtgaagatcc tcgggagaga
    10621 gattttctta aaaccaccct tcacagaatc tatgggaaat tcctaggctt gagagcttac
    10681 atcagaaaac agataaataa tatattttat aggtttattt atgaaacaga gcatcataat
    10741 ggcatagcag agttactgga aatattggga agtataatta atggatttgc cttaccacta
    10801 aaagaagagc acaagatttt cttattgaag gtgttactac ctttgcacaa agtgaaatct
    10861 ctgagtgtct accatcccca gctggcatac tgtgtagtgc agtttttaga aaaggacagc
    10921 accctcacgg aaccagtggt gatggcactt ctcaaatact ggccaaagac tcacagtcca
    10981 aaagaagtaa tgttcttaaa cgaattagaa gagattttag atgtcattga accatcagaa
    11041 tttgtgaaga tcatggaacc cctcttccgg cagttggcca aatgtgtctc cagcccacac
    11101 ttccaggtgg cagagcgagc tctctattac tggaataatg aatacatcat gagtttaatc
    11161 agtgacaacg cagcgaagat tctgcccatc atgtttcctt ccttgtaccg caactcaaag
    11221 acccattgga acaagacaat acatggcttg atatacaacg ccctgaagct cttcatggag
    11281 atgaaccaaa agctatttga tgactgtaca caacagttca aagcagagaa actaaaagag
    11341 aagctaaaaa tgaaagaacg ggaagaagca tgggttaaaa tagaaaatct agccaaagcc
    11401 aatccccagt acacagtgta tagtcaagcc agcaccatga gcattccggt tgcaatggag
    11461 acagatgggc ctttatttga agatgtgcag atgctgagaa agacagtgaa ggacgaggct
    11521 catcaggcac agaaagatcc gaagaaggac cgtcctcttg cactccgcaa gtccgagctg
    11581 cctcaggacc cccacaccaa gaaagccttg gaagctcact gcagggccga tgagctggcc
    11641 tcccaggacg gccgctaggg atccagacat gataagatac attgatgagt ttggacaaac
    11701 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
    11761 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
    11821 gtttcaggtt cagggggagg tggggaggtt ttttaaagca agtaaaacct ctacaaatgt
    11881 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    11941 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    12001 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaccggtc
    12061 gactctaga
//
