LOCUS       sequence-81351-e       15463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ChopV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15463)
  AUTHORS   Kiritani T, Wickersham IR, Seung HS, Shepherd GM
  TITLE     Hierarchical connectivity and connection-specific dynamics in the 
            corticospinal-corticostriatal microcircuit in mouse motor cortex.
  JOURNAL   J Neurosci. 2012 Apr 4;32(14):4992-5001. doi: 
            10.1523/JNEUROSCI.4759-11.2012.
  PUBMED    22492054
REFERENCE   2  (bases 1 to 15463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.4759-11"; journalName: "J Neurosci"; date: 
            "2012-04-4- 4"; volume: "32"; issue: "14"; pages: "4992-5001"
COMMENT     Sequence Label: pRVdG-4ChopV
FEATURES             Location/Qualifiers
     source          1..15463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4695..5621
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      5649..5658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5655..5657,5658..5660,5661..6374)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5700..5721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5961..5980)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6308..6329
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8125..8136
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13135..13153)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13149..13196
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13219..13235)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13219..13235)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13272..13292)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13272..13290)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13311..13327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13311..13327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13324..13346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13335..13351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13359..13365,13366..13383,13384..13389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13404..13425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13542..13559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13713..14301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13793..13812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14472..15263,15264..15332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15095..15114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15333..15437)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggat tatggaggcg ccctgagtgc cgttgggcgc gagctgctat
     4741 ttgtaacgaa cccagtagtc gtcaatggct ctgtacttgt gcctgaggac cagtgttact
     4801 gcgcgggctg gattgagtcg cgtggcacaa acggtgccca aacggcgtcg aacgtgctgc
     4861 aatggcttgc tgctggcttc tccatcctac tgcttatgtt ttacgcctac caaacatgga
     4921 agtcaacctg cggctgggag gagatctatg tgtgcgctat cgagatggtc aaggtgattc
     4981 tcgagttctt cttcgagttt aagaacccgt ccatgctgta tctagccaca ggccaccgcg
     5041 tccagtggtt gcgttacgcc gagtggcttc tcacctgccc ggtcattctc attcacctgt
     5101 caaacctgac gggcttgtcc aacgactaca gcaggcgcac catgggtctg cttgtgtctg
     5161 atattggcac aattgtgtgg ggcgccactt ccgccatggc caccggatac gtcaaggtca
     5221 tcttcttctg cctgggtctg tgttatggtg ctaacacgtt ctttcacgct gccaaggcct
     5281 acatcgaggg ttaccacacc gtgccgaagg gccggtgtcg ccaggtggtg actggcatgg
     5341 cttggctctt cttcgtatca tggggtatgt tccccatcct gttcatcctc ggccccgagg
     5401 gcttcggcgt cctgagcgtg tacggctcca ccgtcggcca caccatcatt gacctgatgt
     5461 cgaagaactg ctggggtctg ctcggccact acctgcgcgt gctgatccac gagcatatcc
     5521 tcatccacgg cgacattcgc aagaccacca aattgaacat tggtggcact gagattgagg
     5581 tcgagacgct ggtggaggac gaggccgagg ctggcgcggt caacaagggc accggcaagc
     5641 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5701 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5761 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     5821 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
     5881 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5941 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6001 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6061 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     6121 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     6181 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6241 tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc
     6301 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6361 agctgtacaa gtaatagcta gcttataaag tgctgggtca tctaagcttt tcagtcgaga
     6421 aaaaaacatt agatcagaag aacaactggc aacacttctc aacctgagac ttacttcaag
     6481 atgctcgatc ctggagaggt ctatgatgac cctattgacc caatcgagtt agaggctgaa
     6541 cccagaggaa cccccattgt ccccaacatc ttgaggaact ctgactacaa tctcaactct
     6601 cctttgatag aagatcctgc tagactaatg ttagaatggt taaaaacagg gaatagacct
     6661 tatcggatga ctctaacaga caattgctcc aggtctttca gagttttgaa agattatttc
     6721 aagaaggtag atttgggttc tctcaaggtg ggcggaatgg ctgcacagtc aatgatttct
     6781 ctctggttat atggtgccca ctctgaatcc aacaggagcc ggagatgtat aacagacttg
     6841 gcccatttct attccaagtc gtcccccata gagaagctgt tgaatctcac gctaggaaat
     6901 agagggctga gaatcccccc agagggagtg ttaagttgcc ttgagagggt tgattatgat
     6961 aatgcatttg gaaggtatct tgccaacacg tattcctctt acttgttctt ccatgtaatc
     7021 accttataca tgaacgccct agactgggat gaagaaaaga ccatcctagc attatggaaa
     7081 gatttaacct cagtggacat cgggaaggac ttggtaaagt tcaaagacca aatatgggga
     7141 ctgctgatcg tgacaaagga ctttgtttac tcccaaagtt ccaattgtct ttttgacaga
     7201 aactacacac ttatgctaaa agatcttttc ttgtctcgct tcaactcctt aatggtcttg
     7261 ctctctcccc cagagccccg atactcagat gacttgatat ctcaactatg ccagctgtac
     7321 attgctgggg atcaagtctt gtctatgtgt ggaaactccg gctatgaagt catcaaaata
     7381 ttggagccat atgtcgtgaa tagtttagtc cagagagcag aaaagtttag gcctctcatt
     7441 cattccttgg gagactttcc tgtatttata aaagacaagg taagtcaact tgaagagacg
     7501 ttcggtccct gtgcaagaag gttctttagg gctctggatc aattcgacaa catacatgac
     7561 ttggtttttg tgtttggctg ttacaggcat tgggggcacc catatataga ttatcgaaag
     7621 ggtctgtcaa aactatatga tcaggttcac cttaaaaaaa tgatagataa gtcctaccag
     7681 gagtgcttag caagcgacct agccaggagg atccttagat ggggttttga taagtactcc
     7741 aagtggtatc tggattcaag attcctagcc cgagaccacc ccttgactcc ttatatcaaa
     7801 acccaaacat ggccacccaa acatattgta gacttggtgg gggatacatg gcacaagctc
     7861 ccgatcacgc agatctttga gattcctgaa tcaatggatc cgtcagaaat attggatgac
     7921 aaatcacatt ctttcaccag aacgagacta gcttcttggc tgtcagaaaa ccgagggggg
     7981 cctgttccta gcgaaaaagt tattatcacg gccctgtcta agccgcctgt caatccccga
     8041 gagtttctga ggtctataga cctcggagga ttgccagatg aagacttgat aattggcctc
     8101 aagccaaagg aacgggaatt gaagattgaa ggtcgattct ttgctctaat gtcatggaat
     8161 ctaagattgt attttgtcat cactgaaaaa ctcttggcca actacatctt gccacttttt
     8221 gacgcgctga ctatgacaga caacctgaac aaggtgttta aaaagctgat cgacagggtc
     8281 accgggcaag ggcttttgga ctattcaagg gtcacatatg catttcacct ggactatgaa
     8341 aagtggaaca accatcaaag attagagtca acagaggatg tattttctgt cctagatcaa
     8401 gtgtttggat tgaagagagt gttttctaga acacacgagt tttttcaaaa ggcctggatc
     8461 tattattcag acagatcaga cctcatcggg ttacgggagg atcaaatata ctgcttagat
     8521 gcgtccaacg gcccaacctg ttggaatggc caggatggcg ggctagaagg cttacggcag
     8581 aagggctgga gtctagtcag cttattgatg atagatagag aatctcaaat caggaacaca
     8641 agaaccaaaa tactagctca aggagacaac caggttttat gtccgacata catgttgtcg
     8701 ccagggctat ctcaagaggg gctcctctat gaattggaga gaatatcaag gaatgcactt
     8761 tcgatataca gagccgtcga ggaaggggca tctaagctag ggctgatcat caagaaagaa
     8821 gagaccatgt gtagttatga cttcctcatc tatggaaaaa cccctttgtt tagaggtaac
     8881 atattggtgc ctgagtccaa aagatgggcc agagtctctt gcgtctctaa tgaccaaata
     8941 gtcaacctcg ccaatataat gtcgacagtg tccaccaatg cgctaacagt ggcacaacac
     9001 tctcaatctt tgatcaaacc gatgagggat tttctgctca tgtcagtaca ggcagtcttt
     9061 cactacctgc tatttagccc aatcttaaag ggaagagttt acaagattct gagcgctgaa
     9121 ggggagagct ttctcctagc catgtcaagg ataatctatc tagatccttc tttgggaggg
     9181 atatctggaa tgtccctcgg aagattccat atacgacagt tctcagaccc tgtctctgaa
     9241 gggttatcct tctggagaga gatctggtta agctcccaag agtcctggat tcacgcgttg
     9301 tgtcaagagg ctggaaaccc agatcttgga gagagaacac tcgagagctt cactcgcctt
     9361 ctagaagatc cgaccacctt aaatatcaga ggaggggcca gtcctaccat tctactcaag
     9421 gatgcaatca gaaaggcttt atatgacgag gtggacaagg tggaaaattc agagtttcga
     9481 gaggcaatcc tgttgtccaa gacccataga gataatttta tactcttctt aatatctgtt
     9541 gagcctctgt ttcctcgatt tctcagtgag ctattcagtt cgtctttttt gggaatcccc
     9601 gagtcaatca ttggattgat acaaaactcc cgaacgataa gaaggcagtt tagaaagagt
     9661 ctctcaaaaa ctttagaaga atccttctac aactcagaga tccacgggat tagtcggatg
     9721 acccagacac ctcagagggt tgggggggtg tggccttgct cttcagagag ggcagatcta
     9781 cttagggaga tctcttgggg aagaaaagtg gtaggcacga cagttcctca cccttctgag
     9841 atgttgggat tacttcccaa gtcctctatt tcttgcactt gtggagcaac aggaggaggc
     9901 aatcctagag tttctgtatc agtactcccg tcctttgatc agtcattttt ttcacgaggc
     9961 cccctaaagg gatacttggg ctcgtccacc tctatgtcga cccagctatt ccatgcatgg
    10021 gaaaaagtca ctaatgttca tgtggtgaag agagctctat cgttaaaaga atctataaac
    10081 tggttcatta ctagagattc caacttggct caagctctaa ttaggaacat tatgtctctg
    10141 acaggccctg atttccctct agaggaggcc cctgtcttca aaaggacggg gtcagccttg
    10201 cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtctg cccgaacctc
    10261 ctctctcata tttctgttag tacagacacc atgtctgatt tgacccaaga cgggaagaac
    10321 tacgatttca tgttccagcc attgatgctt tatgcacaga catggacatc agagctggta
    10381 cagagagaca caaggctaag agactctacg tttcattggc acctccgatg caacaggtgt
    10441 gtgagaccca ttgacgacgt gaccctggag acctctcaga tcttcgagtt tccggatgtg
    10501 tcgaaaagaa tatccagaat ggtttctggg gctgtgcctc acttccagag gcttcccgat
    10561 atccgtctga gaccaggaga ttttgaatct ctaagcggta gagaaaagtc tcaccatatc
    10621 ggatcagctc aggggctctt atactcaatc ttagtggcaa ttcacgactc aggatacaat
    10681 gatggaacca tcttccctgt caacatatac ggcaaggttt cccctagaga ctatttgaga
    10741 gggctcgcaa ggggagtatt gataggatcc tcgatttgct tcttgacaag aatgacaaat
    10801 atcaatatta atagacctct tgaattggtc tcaggggtaa tctcatatat tctcctgagg
    10861 ctagataacc atccctcctt gtacataatg ctcagagaac cgtctcttag aggagagata
    10921 ttttctatcc ctcagaaaat ccccgccgct tatccaacca ctatgaaaga aggcaacaga
    10981 tcaatcttgt gttatctcca acatgtgcta cgctatgagc gagagataat cacggcgtct
    11041 ccagagaatg actggctatg gatcttttca gactttagaa gtgccaaaat gacgtaccta
    11101 tccctcatta cttaccagtc tcatcttcta ctccagaggg ttgagagaaa cctatctaag
    11161 agtatgagag ataacctgcg acaattgagt tctttgatga ggcaggtgct gggcgggcac
    11221 ggagaagata ccttagagtc agacgacaac attcaacgac tgctaaaaga ctctttacga
    11281 aggacaagat gggtggatca agaggtgcgc catgcagcta gaaccatgac tggagattac
    11341 agccccaaca agaaggtgtc ccgtaaggta ggatgttcag aatgggtctg ctctgctcaa
    11401 caggttgcag tctctacctc agcaaacccg gcccctgtct cggagcttga cataagggcc
    11461 ctctctaaga ggttccagaa ccctttgatc tcgggcttga gagtggttca gtgggcaacc
    11521 ggtgctcatt ataagcttaa gcctattcta gatgatctca atgttttccc atctctctgc
    11581 cttgtagttg gggacgggtc aggggggata tcaagggcag tcctcaacat gtttccagat
    11641 gccaagcttg tgttcaacag tcttttagag gtgaatgacc tgatggcttc cggaacacat
    11701 ccactgcctc cttcagcaat catgagggga ggaaatgata tcgtctccag agtgatagat
    11761 cttgactcaa tctgggaaaa accgtccgac ttgagaaact tggcaacctg gaaatacttc
    11821 cagtcagtcc aaaagcaggt caacatgtcc tatgacctca ttatttgcga tgcagaagtt
    11881 actgacattg catctatcaa ccggatcacc ctgttaatgt ccgattttgc attgtctata
    11941 gatggaccac tctatttggt cttcaaaact tatgggacta tgctagtaaa tccaaactac
    12001 aaggctattc aacacctgtc aagagcgttc ccctcggtca cagggtttat cacccaagta
    12061 acttcgtctt tttcatctga gctctacctc cgattctcca aacgagggaa gtttttcaga
    12121 gatgctgagt acttgacctc ttccaccctt cgagaaatga gccttgtgtt attcaattgt
    12181 agcagcccca agagtgagat gcagagagct cgttccttga actatcagga tcttgtgaga
    12241 ggatttcctg aagaaatcat atcaaatcct tacaatgaga tgatcataac tctgattgac
    12301 agtgatgtag aatcttttct agtccacaag atggttgatg atcttgagtt acagagggga
    12361 actctgtcta aagtggctat cattatagcc atcatgatag ttttctccaa cagagtcttc
    12421 aacgtttcca aacccctaac tgacccctcg ttctatccac cgtctgatcc caaaatcctg
    12481 aggcacttca acatatgttg cagtactatg atgtatctat ctactgcttt aggtgacgtc
    12541 cctagcttcg caagacttca cgacctgtat aacagaccta taacttatta cttcagaaag
    12601 caagtcattc gagggaacgt ttatctatct tggagttggt ccaacgacac ctcagtgttc
    12661 aaaagggtag cctgtaattc tagcctgagt ctgtcatctc actggatcag gttgatttac
    12721 aagatagtga agactaccag actcgttggc agcatcaagg atctatccag agaagtggaa
    12781 agacaccttc ataggtacaa caggtggatc accctagagg atatcagatc tagatcatcc
    12841 ctactagact acagttgcct gtgaaccgga tactcctgga agcctgccca tgctaagact
    12901 cttgtgtgat gtatcttgaa aaaaacaaga tcctaaatct gaacctttgg ttgtttgatt
    12961 gtttttctca tttttgttgt ttatttgtta agcgtgggtc ggcatggcat ctccacctcc
    13021 tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc actcggatgg ctaagggagg
    13081 gcggggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
    13141 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
    13201 aaggaggaac tatatccgga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
    13261 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
    13321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    13381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    13441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    13501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    13561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    13621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    13681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    13741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    13801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    13861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    13921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    13981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    14041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    14101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    14161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    14221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    14281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    14341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    14401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    14461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    14521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    14581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    14641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    14701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    14761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    14821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    14881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    14941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    15001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    15061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    15121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    15181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    15241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    15301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    15361 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    15421 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
