LOCUS       sequence-81352-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  multicopy (YEp) vector with a LYS2 marker and a 2-um origin of 
            replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Eriksson P, Thomas LR, Thorburn A, Stillman DJ
  TITLE     pRS yeast vectors with a LYS2 marker.
  JOURNAL   Biotechniques. 2004 Feb;36(2):212-3.
  PUBMED    14989082
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2004-02"; volume: "36(2)"; pages: "212-3"
COMMENT     Sequence Label: pRS327
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(5..23)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    396..443
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
       61 tatgatccaa tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag
      121 tggcagcata tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg
      181 aatgggataa tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata
      241 taagtacgca tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac
      301 aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt
      361 gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat
      421 tctctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc
      481 actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc
      541 gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta
      601 tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt
      661 ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat
      721 agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca
      781 aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt
      841 ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc
      901 ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcggg
//
