LOCUS       sequence-81353-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUK21 (Kan).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUK21-deltaBB
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..36
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20..36
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(50..69)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(51..69)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(76..93)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(76..92)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(85..107)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(392..411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     524..545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(699..718)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     818..840
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(878..896)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atatcgccgt ggcggccgct ctagaactag tggatcgatc cccaattcgc cctatagtga
       61 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      121 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      181 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      241 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
      301 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      361 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
      421 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gtgggccatc gccctgatag
      481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      541 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
      601 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      661 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
      721 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      781 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      841 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctatt
//
