LOCUS       sequence-81354-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T
  TITLE     Reversible dimerization of Aequorea victoria fluorescent proteins 
            increases the dynamic range of FRET-based indicators.
  JOURNAL   ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z.
  PUBMED    20047338
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2010-02-19- 19"; volume: "5"; issue: "2"; 
            pages: "215-22"
COMMENT     Sequence Label: Ateam1.03-nD/nA/pRSETB
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     440..461
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(564..585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(825..844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccggtcacat tcccacaaag gccccactga agatcggagc tgtgcggctg aagaaggacg
       61 gccaaaccga gatggtcgca gtctcaggcg gcactgttga agtgcggcct gaccacgtta
      121 ccattaatgc tcaagccgct gaaacagccg aaggaatcga caaagagaga gcagaagccg
      181 caagacagag ggcccaggag cggctgaact ctcaatccga tgacaccgat attcgccggg
      241 ccgagctggc actgcagagg gccctgaaca gactggacgt ggctgggaag gcaaacgaat
      301 tcatggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      361 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
      421 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      481 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
      541 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      601 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
      661 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
      721 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      781 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      841 agacccgcgc cgaggtgaag ttcgagggcg acacc
//
