LOCUS       sequence-81355-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUK21 (Kan).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUK21-NotI
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     143..159
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     143..159
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(173..192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(174..192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(199..216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(199..215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(208..230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(515..534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     647..668
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(822..841)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atatcgccgt ggcggccgcc agctgaagct tcgtacgctg caggtcgacg gatccccggg
       61 gttaattaag gcgcgccaga tctgtttaaa cgagctcgaa ttcatcgatg atatcagatc
      121 cactagtggc ctatgcggcc gctctagaac tagtggatcg atccccaatt cgccctatag
      181 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
      361 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
      421 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      481 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
      541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggtgggcc atcgccctga
      601 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      661 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
      721 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
      781 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
      841 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      901 ctgctc
//
