LOCUS       sequence-81356-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive 
            Ca2+ indicator with Kd = 15 nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura
            T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
  TITLE     Spontaneous network activity visualized by ultrasensitive Ca(2+) 
            indicators, yellow Cameleon-Nano.
  JOURNAL   Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 
            2010 Aug 8.
  PUBMED    20693999
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1488"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "729-32"
COMMENT     Sequence Label: Yellow Cameleon-Nano15/pcDNA3
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..157
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     301..322
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(380..382,383..385,386..844)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTAVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(425..446)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(686..705)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagatatc gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa tgatgacagc
       61 aaagggggga ggtggctcca agaggcgctg gaagaaaaac ttcattgccg tcagcgctgc
      121 caaccggttc aagaagatct ccagctccgg ggcactggag ctcatggacg gcggcgtgca
      181 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      241 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      301 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      361 caagggtggc agcggtggca tggtgagcaa gggcgaggag ctgttcaccg cggtggtgcc
      421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      481 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
      541 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
      601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      781 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
      841 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atc
//
