LOCUS       sequence-81360-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMDC32 with Zif268:FokI followed by approximately 3kb of filler 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: p35SZ
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(821..841)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(875..899)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(960..1006)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(995..1015)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cttcctcaag tgtcaatgta ccagctttaa tcatttctcc accaatcaaa agctcttcta
       61 cactaagaac agctccatta caattagtga tatgattcaa tcttgtaagc tgagctttgt
      121 agtttccttt gaaatgacca ctcacaaaca agaacttaaa ttctgttact gaagatggat
      181 agactttcca ccattcatta gggttgatat gcttgtttct tgtttgattc tcttcgacat
      241 atctttgcat ttcatctgct tgaccaattg gaagattata acctcctgaa tatgccttag
      301 tatcaacgat cacaccgtaa tcaataggag atccgacagt ataaattgct ccgtctggtt
      361 tccttgatcc acccaaatgt ttaccacgat aaccataaac cttcataaag aactccatca
      421 ccttcatttc aaggattcta tcctgagttg aatttcttgc gatttcaatc aattcaatat
      481 attcatgtgg cacatatttc aatttatgtc taagttcaga tttcttctct tccaattcag
      541 atttcactag ttccttctga cgaagatgga tctttgtatg acgcttcctt tcatcacttc
      601 ttgcgaactt acgaccacag atgtcacatg cgaatggttt ctcaccagta tgggttctga
      661 tatgtgtagt caaatgatcg ctacgactaa agttcctcat acagattcta cactggaaag
      721 gcttctgtcc tgtgtggatt ctaatatgcc tagtgagttc gtctgaccta ctaaaacgtc
      781 tatcacaaga ctcaactgga caagcgtaag gcaattcaag aacctttctc tttttctttg
      841 gaggggagga agccatggtg aagggcgaat tcggagcctg cttttttgta caaacttgtt
      901 tgatagcttg gcgcgcctcg agggggggcc cggtacccgg ggatcctcta gagtcgaggt
      961 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     1021 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     1081 ggttggaacg
//
