LOCUS       sequence-81361-e       17192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 17192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L
FEATURES             Location/Qualifiers
     source          1..17192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1452..1461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1458..1460,1461..1463,1464..2177)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1458..1460,1461..1463,1464..2174)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1503..1524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1764..1783)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2111..2132
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5528..6226
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             6251..6271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6275..6295
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6299..6319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6404..6413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7364..7373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7370..8101
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             9853..9864
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14864..14882)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      14878..14925
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(14948..14964)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14948..14964)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(15001..15021)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15001..15019)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15040..15056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15040..15056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15053..15075)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15064..15080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15088..15094,15095..15112,15113..15118))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15133..15154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15271..15288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15442..16030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15522..15541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16201..16992,16993..17061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16824..16843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17062..17166)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 ccgggaaagc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1501 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1561 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1621 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1681 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1741 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1801 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1861 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1921 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1981 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2041 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2101 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2161 acgagctgta caagtaagct agggaagttg aataacaaaa tgccggaaat ctacggattg
     2221 tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca aacatggatg
     2281 ccgacaagat tgtattcaaa gtcaataatc aggtggtctc tttgaagcct gagattatcg
     2341 tggatcaata tgagtacaag taccctgcca tcaaagattt gaaaaagccc tgtataactc
     2401 taggaaaggc tcccgattta aataaagcat acaagtcagt tttgtcaggc atgagcgccg
     2461 ccaaacttaa tcctgacgat gtatgttcct atttggcagc ggcaatgcag ttttttgagg
     2521 ggacatgtcc ggaagactgg accagctatg gaattgtgat tgcacgaaaa ggagataaga
     2581 tcaccccagg ttctctggtg gagataaaac gtactgatgt agaagggaat tgggctctga
     2641 caggaggcat ggaactgaca agagacccca ctgtccctga gcatgcgtcc ttagtcggtc
     2701 ttctcttgag tctgtatagg ttgagcaaaa tatccgggca aaacactggt aactataaga
     2761 caaacattgc agacaggata gagcagattt ttgagacagc cccttttgtt aaaatcgtgg
     2821 aacaccatac tctaatgaca actcacaaaa tgtgtgctaa ttggagtact ataccaaact
     2881 tcagattttt ggccggaacc tatgacatgt ttttctcccg gattgagcat ctatattcag
     2941 caatcagagt gggcacagtt gtcactgctt atgaagactg ttcaggactg gtatcattta
     3001 ctgggttcat aaaacaaatc aatctcaccg ctagagaggc aatactatat ttcttccaca
     3061 agaactttga ggaagagata agaagaatgt ttgagccagg gcaggagaca gctgttcctc
     3121 actcttattt catccacttc cgttcactag gcttgagtgg gaaatctcct tattcatcaa
     3181 atgctgttgg tcacgtgttc aatctcattc actttgtagg atgctatatg ggtcaagtca
     3241 gatccctaaa tgcaacggtt attgctgcat gtgctcctca tgaaatgtct gttctagggg
     3301 gctatctggg agaggaattc ttcgggaaag ggacatttga aagaagattc ttcagagatg
     3361 agaaagaact tcaagaatac gaggcggctg aactgacaaa gactgacgta gcactggcag
     3421 atgatggaac tgtcaactct gacgacgagg actacttttc aggtgaaacc agaagtccgg
     3481 aggctgttta tactcgaatc atgatgaatg gaggtcgact aaagagatct cacatacgga
     3541 gatatgtctc agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc gagtttctaa
     3601 acaagacata ttcgagtgac tcataagaag ttgaataaca aaatgccgga aatctacgga
     3661 ttgtgtatat ccatcatgaa aaaaactaac acccctcctt tcgaaccatc ccaaacatga
     3721 gcaagatctt tgtcaatcct agtgctatta gagccggtct ggccgatctt gagatggctg
     3781 aagaaactgt tgatctgatc aatagaaata tcgaagacaa tcaggctcat ctccaagggg
     3841 aacccataga ggtggacaat ctccctgagg atatggggcg acttcacctg gatgatggaa
     3901 aatcgcccaa ccatggtgag atagccaagg tgggagaagg caagtatcga gaggactttc
     3961 agatggatga aggagaggat cctagcttcc tgttccagtc atacctggaa aatgttggag
     4021 tccaaatagt cagacaaatg aggtcaggag agagatttct caagatatgg tcacagaccg
     4081 tagaagagat tatatcctat gtcgcggtca actttcccaa ccctccagga aagtcttcag
     4141 aggataaatc aacccagact actggccgag agctcaagaa ggagacaaca cccactcctt
     4201 ctcagagaga aagccaatca tcgaaagcca ggatggcggc tcaaattgct tctggccctc
     4261 cagcccttga atggtcggct accaatgaag aggatgatct atcagtggag gctgagatcg
     4321 ctcaccagat tgcagaaagt ttctccaaaa aatataagtt tccctctcga tcctcaggga
     4381 tactcttgta taattttgag caattgaaaa tgaaccttga tgatatagtt aaagaggcaa
     4441 aaaatgtacc aggtgtgacc cgtttagccc atgacgggtc caaactcccc ctaagatgtg
     4501 tactgggatg ggtcgctttg gccaactcta agaaattcca gttgttagtc gaatccgaca
     4561 agctgagtaa aatcatgcaa gatgacttga atcgctatac atcttgctaa ccgaacctct
     4621 cccctcagtc cctctagaca ataaaatccg agatgtccca aagtcaacat gaaaaaaaca
     4681 ggcaacacca ctgataaaat gaacctccta cgtaagatag tgaaaaaccg cagggacgag
     4741 gacactcaaa aatcctctcc cgcgtcagcc cctctggatg acgatgactt gtggcttcca
     4801 ccccctgaat acgtcccgct gaaagaactt acaggcaaga agaacatgag gaacttttgt
     4861 atcaacggaa gggttaaagt gtgtagcccg aatggttact cgttcaggat cctgcggcac
     4921 attctgaaat cattcgacga gatatattct gggaatcata ggatgatcgg gttagtcaaa
     4981 gtggttattg gactggcttt gtcaggatct ccagtccctg agggcctgaa ctgggtatac
     5041 aaattgagga gaacctttat cttccagtgg gctgattcca ggggccctct tgaaggggag
     5101 gagttggaat actctcagga gatcacttgg gatgatgata ctgagttcgt cggattgcaa
     5161 ataagagtga ttgcaaaaca gtgtcatatc cagggcagag tctggtgtat caacatgaac
     5221 ccgagagcat gtcaactatg gtctgacatg tctcttcaga cacaaaggtc cgaagaggac
     5281 aaagattcct ctctgcttct agaataatca gattatatcc cgcaaattta tcacttgttt
     5341 acctctggag gagagaacat atgggctcaa ctccaaccct tgggagcaat ataacaaaaa
     5401 acatgttatg gtgccattaa accgctgcat ttcatcaaag tcaagttgat tacctttaca
     5461 ttttgatcct cttggatgtg aaaaaaacta ttaacatccc tcaaaagacc ccgggaaagc
     5521 cgccaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     5581 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     5641 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     5701 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     5761 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     5821 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     5881 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     5941 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     6001 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     6061 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     6121 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     6181 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattccg gactcagatc
     6241 tcgagctgat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc
     6301 aaaaaagaag agaaaggtag gatccaccgg atctagataa ttaattaacg tcctttcaac
     6361 gatccaagtc catgaaaaaa actaacaccc ctcccgtacg gccgccacca tggacgtggt
     6421 gaatcagctg gtggctgggg gtcagttccg ggtggtcaag gagccccttg gcttcgtgaa
     6481 ggtgctgcag tgggtctttg ccatcttcgc ctttgctacg tgcggcagct acaccggaga
     6541 gcttcggctg agcgtggagt gtgccaacaa gacggagagt gccctcaaca tcgaagtcga
     6601 atttgagtac ccattcaggc tgcaccaagt gtactttgat gcaccctcct gcgttaaagg
     6661 gggcactacc aagatcttcc tagttggtga ctactcctcc tcgggtgaat tctttgtcac
     6721 cgtggctgtg tttgccttcc tctactccat gggggccctg gccacctaca tcttcctgca
     6781 gaacaagtac cgagagaaca acaaagggcc aatgatggac ttcctggcca cagcagtgtt
     6841 cgctttcatg tggctagtta gctcatccgc ctgggccaaa ggcctgtccg atgtgaagat
     6901 ggccactgac ccagagaaca ttatcaagga gatgcctatg tgccgccaga caggaaacac
     6961 atgcaaggaa ctgagggacc ctgtgacttc aggactcaac acctcggtgg tgtttggctt
     7021 cctgaacctg gtgctctggg ttggcaacct atggttcgtg ttcaaggaga caggctgggc
     7081 cgccccattc atgcgcgcac ctccaggcgc cccagaaaag caaccagctc ctggcgatgc
     7141 ctacggcgat gcgggctatg ggcagggccc cggaggctat gggccccagg actcctacgg
     7201 gcctcagggt ggttatcaac ccgattacgg gcagccagcc agcggcggtg gcggtggcta
     7261 cgggcctcag ggcgactatg ggcagcaagg ctacggccaa cagggtgcgc ccacctcctt
     7321 ctccaatcag atgggcggag gccctgggat ccaccggccg gtcgccacca tggtgtctaa
     7381 gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
     7441 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     7501 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     7561 cagcttcatg tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt
     7621 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     7681 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     7741 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     7801 ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaaccgacat
     7861 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc
     7921 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga
     7981 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
     8041 atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg agctgtacaa
     8101 gtaagctagc ttataaagtg ctgggtcatc taagcttttc agtcgagaaa aaaacattag
     8161 atcagaagaa caactggcaa cacttctcaa cctgagactt acttcaagat gctcgatcct
     8221 ggagaggtct atgatgaccc tattgaccca atcgagttag aggctgaacc cagaggaacc
     8281 cccattgtcc ccaacatctt gaggaactct gactacaatc tcaactctcc tttgatagaa
     8341 gatcctgcta gactaatgtt agaatggtta aaaacaggga atagacctta tcggatgact
     8401 ctaacagaca attgctccag gtctttcaga gttttgaaag attatttcaa gaaggtagat
     8461 ttgggttctc tcaaggtggg cggaatggct gcacagtcaa tgatttctct ctggttatat
     8521 ggtgcccact ctgaatccaa caggagccgg agatgtataa cagacttggc ccatttctat
     8581 tccaagtcgt cccccataga gaagctgttg aatctcacgc taggaaatag agggctgaga
     8641 atccccccag agggagtgtt aagttgcctt gagagggttg attatgataa tgcatttgga
     8701 aggtatcttg ccaacacgta ttcctcttac ttgttcttcc atgtaatcac cttatacatg
     8761 aacgccctag actgggatga agaaaagacc atcctagcat tatggaaaga tttaacctca
     8821 gtggacatcg ggaaggactt ggtaaagttc aaagaccaaa tatggggact gctgatcgtg
     8881 acaaaggact ttgtttactc ccaaagttcc aattgtcttt ttgacagaaa ctacacactt
     8941 atgctaaaag atcttttctt gtctcgcttc aactccttaa tggtcttgct ctctccccca
     9001 gagccccgat actcagatga cttgatatct caactatgcc agctgtacat tgctggggat
     9061 caagtcttgt ctatgtgtgg aaactccggc tatgaagtca tcaaaatatt ggagccatat
     9121 gtcgtgaata gtttagtcca gagagcagaa aagtttaggc ctctcattca ttccttggga
     9181 gactttcctg tatttataaa agacaaggta agtcaacttg aagagacgtt cggtccctgt
     9241 gcaagaaggt tctttagggc tctggatcaa ttcgacaaca tacatgactt ggtttttgtg
     9301 tttggctgtt acaggcattg ggggcaccca tatatagatt atcgaaaggg tctgtcaaaa
     9361 ctatatgatc aggttcacct taaaaaaatg atagataagt cctaccagga gtgcttagca
     9421 agcgacctag ccaggaggat ccttagatgg ggttttgata agtactccaa gtggtatctg
     9481 gattcaagat tcctagcccg agaccacccc ttgactcctt atatcaaaac ccaaacatgg
     9541 ccacccaaac atattgtaga cttggtgggg gatacatggc acaagctccc gatcacgcag
     9601 atctttgaga ttcctgaatc aatggatccg tcagaaatat tggatgacaa atcacattct
     9661 ttcaccagaa cgagactagc ttcttggctg tcagaaaacc gaggggggcc tgttcctagc
     9721 gaaaaagtta ttatcacggc cctgtctaag ccgcctgtca atccccgaga gtttctgagg
     9781 tctatagacc tcggaggatt gccagatgaa gacttgataa ttggcctcaa gccaaaggaa
     9841 cgggaattga agattgaagg tcgattcttt gctctaatgt catggaatct aagattgtat
     9901 tttgtcatca ctgaaaaact cttggccaac tacatcttgc cactttttga cgcgctgact
     9961 atgacagaca acctgaacaa ggtgtttaaa aagctgatcg acagggtcac cgggcaaggg
    10021 cttttggact attcaagggt cacatatgca tttcacctgg actatgaaaa gtggaacaac
    10081 catcaaagat tagagtcaac agaggatgta ttttctgtcc tagatcaagt gtttggattg
    10141 aagagagtgt tttctagaac acacgagttt tttcaaaagg cctggatcta ttattcagac
    10201 agatcagacc tcatcgggtt acgggaggat caaatatact gcttagatgc gtccaacggc
    10261 ccaacctgtt ggaatggcca ggatggcggg ctagaaggct tacggcagaa gggctggagt
    10321 ctagtcagct tattgatgat agatagagaa tctcaaatca ggaacacaag aaccaaaata
    10381 ctagctcaag gagacaacca ggttttatgt ccgacataca tgttgtcgcc agggctatct
    10441 caagaggggc tcctctatga attggagaga atatcaagga atgcactttc gatatacaga
    10501 gccgtcgagg aaggggcatc taagctaggg ctgatcatca agaaagaaga gaccatgtgt
    10561 agttatgact tcctcatcta tggaaaaacc cctttgttta gaggtaacat attggtgcct
    10621 gagtccaaaa gatgggccag agtctcttgc gtctctaatg accaaatagt caacctcgcc
    10681 aatataatgt cgacagtgtc caccaatgcg ctaacagtgg cacaacactc tcaatctttg
    10741 atcaaaccga tgagggattt tctgctcatg tcagtacagg cagtctttca ctacctgcta
    10801 tttagcccaa tcttaaaggg aagagtttac aagattctga gcgctgaagg ggagagcttt
    10861 ctcctagcca tgtcaaggat aatctatcta gatccttctt tgggagggat atctggaatg
    10921 tccctcggaa gattccatat acgacagttc tcagaccctg tctctgaagg gttatccttc
    10981 tggagagaga tctggttaag ctcccaagag tcctggattc acgcgttgtg tcaagaggct
    11041 ggaaacccag atcttggaga gagaacactc gagagcttca ctcgccttct agaagatccg
    11101 accaccttaa atatcagagg aggggccagt cctaccattc tactcaagga tgcaatcaga
    11161 aaggctttat atgacgaggt ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg
    11221 ttgtccaaga cccatagaga taattttata ctcttcttaa tatctgttga gcctctgttt
    11281 cctcgatttc tcagtgagct attcagttcg tcttttttgg gaatccccga gtcaatcatt
    11341 ggattgatac aaaactcccg aacgataaga aggcagttta gaaagagtct ctcaaaaact
    11401 ttagaagaat ccttctacaa ctcagagatc cacgggatta gtcggatgac ccagacacct
    11461 cagagggttg ggggggtgtg gccttgctct tcagagaggg cagatctact tagggagatc
    11521 tcttggggaa gaaaagtggt aggcacgaca gttcctcacc cttctgagat gttgggatta
    11581 cttcccaagt cctctatttc ttgcacttgt ggagcaacag gaggaggcaa tcctagagtt
    11641 tctgtatcag tactcccgtc ctttgatcag tcattttttt cacgaggccc cctaaaggga
    11701 tacttgggct cgtccacctc tatgtcgacc cagctattcc atgcatggga aaaagtcact
    11761 aatgttcatg tggtgaagag agctctatcg ttaaaagaat ctataaactg gttcattact
    11821 agagattcca acttggctca agctctaatt aggaacatta tgtctctgac aggccctgat
    11881 ttccctctag aggaggcccc tgtcttcaaa aggacggggt cagccttgca taggttcaag
    11941 tctgccagat acagcgaagg agggtattct tctgtctgcc cgaacctcct ctctcatatt
    12001 tctgttagta cagacaccat gtctgatttg acccaagacg ggaagaacta cgatttcatg
    12061 ttccagccat tgatgcttta tgcacagaca tggacatcag agctggtaca gagagacaca
    12121 aggctaagag actctacgtt tcattggcac ctccgatgca acaggtgtgt gagacccatt
    12181 gacgacgtga ccctggagac ctctcagatc ttcgagtttc cggatgtgtc gaaaagaata
    12241 tccagaatgg tttctggggc tgtgcctcac ttccagaggc ttcccgatat ccgtctgaga
    12301 ccaggagatt ttgaatctct aagcggtaga gaaaagtctc accatatcgg atcagctcag
    12361 gggctcttat actcaatctt agtggcaatt cacgactcag gatacaatga tggaaccatc
    12421 ttccctgtca acatatacgg caaggtttcc cctagagact atttgagagg gctcgcaagg
    12481 ggagtattga taggatcctc gatttgcttc ttgacaagaa tgacaaatat caatattaat
    12541 agacctcttg aattggtctc aggggtaatc tcatatattc tcctgaggct agataaccat
    12601 ccctccttgt acataatgct cagagaaccg tctcttagag gagagatatt ttctatccct
    12661 cagaaaatcc ccgccgctta tccaaccact atgaaagaag gcaacagatc aatcttgtgt
    12721 tatctccaac atgtgctacg ctatgagcga gagataatca cggcgtctcc agagaatgac
    12781 tggctatgga tcttttcaga ctttagaagt gccaaaatga cgtacctatc cctcattact
    12841 taccagtctc atcttctact ccagagggtt gagagaaacc tatctaagag tatgagagat
    12901 aacctgcgac aattgagttc tttgatgagg caggtgctgg gcgggcacgg agaagatacc
    12961 ttagagtcag acgacaacat tcaacgactg ctaaaagact ctttacgaag gacaagatgg
    13021 gtggatcaag aggtgcgcca tgcagctaga accatgactg gagattacag ccccaacaag
    13081 aaggtgtccc gtaaggtagg atgttcagaa tgggtctgct ctgctcaaca ggttgcagtc
    13141 tctacctcag caaacccggc ccctgtctcg gagcttgaca taagggccct ctctaagagg
    13201 ttccagaacc ctttgatctc gggcttgaga gtggttcagt gggcaaccgg tgctcattat
    13261 aagcttaagc ctattctaga tgatctcaat gttttcccat ctctctgcct tgtagttggg
    13321 gacgggtcag gggggatatc aagggcagtc ctcaacatgt ttccagatgc caagcttgtg
    13381 ttcaacagtc ttttagaggt gaatgacctg atggcttccg gaacacatcc actgcctcct
    13441 tcagcaatca tgaggggagg aaatgatatc gtctccagag tgatagatct tgactcaatc
    13501 tgggaaaaac cgtccgactt gagaaacttg gcaacctgga aatacttcca gtcagtccaa
    13561 aagcaggtca acatgtccta tgacctcatt atttgcgatg cagaagttac tgacattgca
    13621 tctatcaacc ggatcaccct gttaatgtcc gattttgcat tgtctataga tggaccactc
    13681 tatttggtct tcaaaactta tgggactatg ctagtaaatc caaactacaa ggctattcaa
    13741 cacctgtcaa gagcgttccc ctcggtcaca gggtttatca cccaagtaac ttcgtctttt
    13801 tcatctgagc tctacctccg attctccaaa cgagggaagt ttttcagaga tgctgagtac
    13861 ttgacctctt ccacccttcg agaaatgagc cttgtgttat tcaattgtag cagccccaag
    13921 agtgagatgc agagagctcg ttccttgaac tatcaggatc ttgtgagagg atttcctgaa
    13981 gaaatcatat caaatcctta caatgagatg atcataactc tgattgacag tgatgtagaa
    14041 tcttttctag tccacaagat ggttgatgat cttgagttac agaggggaac tctgtctaaa
    14101 gtggctatca ttatagccat catgatagtt ttctccaaca gagtcttcaa cgtttccaaa
    14161 cccctaactg acccctcgtt ctatccaccg tctgatccca aaatcctgag gcacttcaac
    14221 atatgttgca gtactatgat gtatctatct actgctttag gtgacgtccc tagcttcgca
    14281 agacttcacg acctgtataa cagacctata acttattact tcagaaagca agtcattcga
    14341 gggaacgttt atctatcttg gagttggtcc aacgacacct cagtgttcaa aagggtagcc
    14401 tgtaattcta gcctgagtct gtcatctcac tggatcaggt tgatttacaa gatagtgaag
    14461 actaccagac tcgttggcag catcaaggat ctatccagag aagtggaaag acaccttcat
    14521 aggtacaaca ggtggatcac cctagaggat atcagatcta gatcatccct actagactac
    14581 agttgcctgt gaaccggata ctcctggaag cctgcccatg ctaagactct tgtgtgatgt
    14641 atcttgaaaa aaacaagatc ctaaatctga acctttggtt gtttgattgt ttttctcatt
    14701 tttgttgttt atttgttaag cgtgggtcgg catggcatct ccacctcctc gcggtccgac
    14761 ctgggcatcc gaaggaggac gcacgtccac tcggatggct aagggagggg cggggatccg
    14821 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
    14881 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
    14941 atatccggat ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
    15001 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    15061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    15121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    15181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    15241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    15301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    15361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    15421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    15481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    15541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    15601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    15661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    15721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    15781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    15841 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    15901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    15961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    16021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    16081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    16141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    16201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    16261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    16321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    16381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    16441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    16501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    16561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    16621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    16681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    16741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    16801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    16861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    16921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    16981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    17041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    17101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    17161 tccgcgcaca tttccccgaa aagtgccacc tg
//
