LOCUS       sequence-81362-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Phamret, a monomeric photoconvertible 
            fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Matsuda T, Miyawaki A, Nagai T
  TITLE     Direct measurement of protein dynamics inside cells using a 
            rationally designed photoconvertible protein.
  JOURNAL   Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 
            2008 Mar 16.
  PUBMED    18345008
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1193"; journalName: "Nat Methods"; date: "2008-04"; 
            volume: "5"; issue: "4"; pages: "339-45"
COMMENT     Sequence Label: Phamret/pcDNA3
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     455..474
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     714..735
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atagggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc
       61 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      121 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt ggctcaggta
      181 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      241 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
      301 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      361 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      421 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      481 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      541 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg tagctgaagg tggtcacgag
      601 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      661 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      721 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tggtaccggc
      781 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      841 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
//
